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PMID: 392505 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Attractants and repellents control demethylation of methylated chemotaxis proteins in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 11 ·1979-11-00 ·Pages 5544-8

Toews ML, Goy MF, Springer MS, Adler J

Abstract

A group of methylated proteins, the methyl-accepting chemotaxis proteins (MCP), has been shown to play a central role in bacterial chemotaxis. Both methylation and demethylation of MCP occur continuously in the absence of added stimuli; these two processes are in balance such that a basal level of methylation is maintained. Attractants cause the methylation level to increase to a new value, whereas repellents bring about a decrease in level. Therefore, attractants and repellents must somehow perturb the balance between methylation and demethylation of MCP. In this report the effect of attractants on demethylation of MCP was monitored in two ways: (i) by following the disappearance of [methyl-3H]MCP and (ii) by measuring formation of [3H]methanol, the product of MCP demethylation. Both methods showed that addition of attractants causes a transient inhibition of MCP demethylation. Repellent addition has previously been shown to stimulate MCP demethylation. It is therefore concluded that control of demethylation plays a crucial role in changing the level of methylation of MCP in response to attractants and repellents.

MeSH Terms
Bacterial Proteins/metabolism Chemoreceptor Cells/metabolism Chemotaxis Escherichia coli/physiology Kinetics Membrane Proteins/metabolism Methylation
Chemicals
Bacterial Proteins Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Toews M L
Goy M F
Springer M S
Adler J
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.11.5544
Published
1979-11-00
Pages
5544-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411685
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.11.5544 PMC Full Text View journal details
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