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PMID: 3919139 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of an epibody. An antiidiotype that reacts with both the idiotype of rheumatoid factors (RF) and the antigen recognized by RF.

The Journal of experimental medicine ·Vol. 161 ·No. 2 ·1985-02-01 ·Pages 323-31

Chen PP, Fong S, Houghten RA, Carson DA

Abstract

Recently, an antiidiotype to human monoclonal IgM anti-IgG autoantibodies (rheumatoid factors) was found to react also with human IgG. This peculiar antiidiotype was called an 'epibody'. We describe the induction of a similar epibody by immunization with a synthetic peptide (corresponding to one hypervariable region of the IgM-RF Glo). The results confirm the existence of epibodies, and provide the possible molecular basis of the epibody phenomenon.

MeSH Terms
Animals Antibodies, Anti-Idiotypic/analysis,biosynthesis,immunology Antigen-Antibody Reactions Antigens/immunology Autoantigens/immunology Chromatography, Affinity Immunoglobulin G/immunology Immunoglobulin Idiotypes/immunology Immunoglobulin Light Chains/immunology Peptides/immunology,pharmacology Rabbits Rheumatoid Factor/immunology
Chemicals
Antibodies, Anti-Idiotypic Antigens Autoantigens Immunoglobulin G Immunoglobulin Idiotypes Immunoglobulin Light Chains Peptides rheumatoid arthritis nuclear antigen Rheumatoid Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chen P P
Fong S
Houghten R A
Carson D A
References (19)
19 references, click to expand
  1. The internal image of IgG in cross-reactive anti-idiotypic antibodies against human rheumatoid factors.
    J Immunol. 1983 Aug;131(2):719-24 PMID: 6863929
  2. Immunochemical studies on the tobacco mosaic virus protein. VII. The binding of octanoylated peptides of the tobacco mosaic virus protein with antibodies to the whole protein.
    Biochemistry. 1968 Apr;7(4):1261-4 PMID: 5663399
  3. Induction of rheumatoid antibodies in the mouse. Regulated production of autoantibody in the secondary humoral response.
    J Exp Med. 1983 Aug 1;158(2):529-45 PMID: 6350525
  4. Rheumatoid factors and secondary immune responses in the mouse. I. Frequent occurrence of hybridomas secreting IgM anti-IgG1 autoantibodies after immunization with protein antigens.
    Eur J Immunol. 1983 Nov;13(11):890-4 PMID: 6605860
  5. Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.
    J Immunol. 1982 Mar;128(3):1176-80 PMID: 6173424
  6. Hypervariable regions, idiotypy, and the antibody-combining site.
    Adv Immunol. 1975;20:1-40 PMID: 47218
  7. Individual Antigenic Specificity of Isolated Antibodies.
    Science. 1963 Jun 14;140(3572):1218-9 PMID: 17837506
  8. Anti-hypervariable region antibody induced by a defined peptide: an approach for studying the structural correlates of idiotypes.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1784-8 PMID: 6200884
  9. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  10. A common idiotope on human rheumatoid factors identified by a hybridoma antibody.
    Mol Immunol. 1983 Oct;20(10):1081-7 PMID: 6201726
  11. Preferential association of kappa IIIb light chains with monoclonal human IgM kappa autoantibodies.
    J Immunol. 1983 Sep;131(3):1322-5 PMID: 6193185
  12. Rheumatoid factors and secondary immune responses in the mouse. II. Incidence, kinetics and induction mechanisms.
    Eur J Immunol. 1983 Nov;13(11):895-9 PMID: 6641787
  13. Immunological relationships among the myeloma proteins.
    J Exp Med. 1955 Jan 1;101(1):85-108 PMID: 13211928
  14. The amino acid sequence of the variable regions of the light chains from two idiotypically cross reactive IgM anti-gamma globulins.
    Ann Immunol (Paris). 1976 Jun-Jul;127(3-4):261-71 PMID: 60899
  15. Anti-immunoglobulin antibodies. III. Properties of sequential anti-idiotypic antibodies to heterologous anti-gamma globulins. Detection of reactivity of anti-idiotype antibodies with epitopes of Fc fragments (homobodies) and with epitopes and idiotopes (epibodies).
    J Exp Med. 1982 Oct 1;156(4):986-99 PMID: 6185609
  16. Delineation of a cross-reactive idiotype on human autoantibodies with antibody against a synthetic peptide.
    J Exp Med. 1984 May 1;159(5):1502-11 PMID: 6201589
  17. New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates.
    Biochemistry. 1979 Feb 20;18(4):690-3 PMID: 84683
  18. Enhancing antibody: a novel component of the immune response.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3828-32 PMID: 6179088
  19. Complete amino acid sequence of variable domains from two monoclonal human anti-gamma globulins of the Wa cross-idiotypic group: suggestion that the J segments are involved in the structural correlate of the idiotype.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3799-803 PMID: 6167990
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.161.2.323
Published
1985-02-01
Pages
323-31
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187561
Subset
IM
Grants
NIA NIH HHS · AG04100 · United States
NIADDK NIH HHS · AM21175 · United States
NIADDK NIH HHS · AM25443 · United States
External Links
PubMed source DOI: 10.1084/jem.161.2.323 PMC Full Text View journal details
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