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PMID: 3918001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A mutation in Escherichia coli K-12 results in a requirement for thiamine and a decrease in L-serine deaminase activity.

Journal of bacteriology ·Vol. 161 ·No. 1 ·1985-01-00 ·Pages 272-6

Newman EB, Miller B, Colebrook LD, Walker C

Abstract

Mutants of Escherichia coli K-12 deficient in L-serine deaminase (L-SD) activity have been isolated. These strains required thiamine and grew normally when it was provided. The decrease in L-SD activity caused no obvious metabolic deficiency. A study of revertants and transductants showed that a single mutation was responsible for the thiamine requirement and for the decrease in L-SD activity.

MeSH Terms
Drug Resistance, Microbial Escherichia coli/enzymology,genetics,growth & development Glycine/metabolism L-Serine Dehydratase/metabolism Leucine/metabolism Pyruvates/metabolism Pyruvic Acid Serine/metabolism Thiamine/physiology Transduction, Genetic beta-Galactosidase/biosynthesis
Chemicals
Pyruvates Serine Pyruvic Acid beta-Galactosidase L-Serine Dehydratase Leucine Glycine Thiamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Newman E B
Miller B
Colebrook L D
Walker C
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.1.272-276.1985
Published
1985-01-00
Pages
272-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214867
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.161.1.272-276.1985 PMC Full Text View journal details
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