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PMID: 3907847 Published · ppublish English Journal Article

Characterization of dendritic cells, isolated from normal and stimulated lymph nodes of the rat.

Cell and tissue research ·Vol. 242 ·No. 3 ·1985-00-00 ·Pages 469-74

Kamperdijk EW, Kapsenberg ML, van den Berg M, Hoefsmit EC

Abstract

Non-lymphoid dendritic cells were isolated from normal and paratyphoid vaccine-stimulated lymph nodes draining the rat skin. They were studied using enzymecytochemical, immunocytochemical and electron-microscopical methods. These cells had an irregular outline and an eccentrically situated nucleus. All showed acid phosphatase activity in a central area and expressed Ia antigen on the plasma membrane. Birbeck granules were exclusively present in dendritic cells isolated from lymph nodes in the induction phase of the immune response. This observation concurs with the presence of Birbeck granules in interdigitating cells in situ during the same period of the immune response. It is concluded that the dendritic cells are the in-vitro equivalents of the non-actively phagocytizing population of interdigitating cells.

MeSH Terms
Acid Phosphatase/metabolism Animals Cell Separation/methods Immunoenzyme Techniques Lymph Nodes/cytology,enzymology,ultrastructure Male Microscopy, Electron Rats Rats, Inbred Strains
Chemicals
Acid Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kamperdijk E W
Kapsenberg M L
van den Berg M
Hoefsmit E C
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32 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00225411
Published
1985-00-00
Pages
469-74
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00225411 View journal details
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