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PMID: 3899940 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Attachment of staphylococci and streptococci on fibronectin, fibronectin fragments, and fibrinogen bound to a solid phase.

Infection and immunity ·Vol. 50 ·No. 1 ·1985-10-00 ·Pages 77-81

Kuusela P, Vartio T, Vuento M, Myhre EB

Abstract

The attachment of Staphylococcus aureus (Cowan I) and two strains of group A and G streptococci on glass cover slips coated with fibronectin, fibronectin fragments, or fibrinogen was studied. The attachment was quantitated by counting the attached bacteria on glass surfaces coated with a similar molarity of the proteins. Fibronectin was a more effective attachment factor than fibrinogen for staphylococci, while group G streptococci attached better on fibrinogen- than on fibronectin-coated cover slips. In this system, group A streptococci bound almost exclusively to substrate-bound fibrinogen. Attachment experiments involving the use of staphylococci pretreated with soluble fibronectin or fibrinogen revealed that bacterium-bound fibronectin and fibrinogen were able to enhance the adherence on cover slips coated with fibronectin. The 30-kilodalton NH2-terminal and the 120- to 140-kilodalton COOH-terminal fragments of fibronectin, both of which contain bacterial binding sites, mediated the staphylococcal attachment, suggesting that both parts of the molecule are involved in the attachment mediated by fibronectin.

MeSH Terms
Adhesiveness Fibrinogen Fibronectins Glass Peptide Fragments Protein Binding Solubility Staphylococcus Streptococcus Streptococcus pyogenes Time Factors
Chemicals
Fibronectins Peptide Fragments Fibrinogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuusela P
Vartio T
Vuento M
Myhre E B
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37 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.50.1.77-81.1985
Published
1985-10-00
Pages
77-81
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262138
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.50.1.77-81.1985 PMC Full Text View journal details
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