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PMID: 3891737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Convergently functional, Rho-independent terminator in Salmonella typhimurium.

Journal of bacteriology ·Vol. 163 ·No. 1 ·1985-07-00 ·Pages 362-8

Carlomagno MS, Riccio A, Bruni CB

Abstract

A typical Rho-independent terminator of transcription was found at the end of the histidine operon of Salmonella typhimurium. This site is used to terminate, in addition to the his operon mRNA, a 1,200-nucleotide RNA of unknown function transcribed on the opposite strand. The efficiency of termination of transcription at this site was investigated in vivo and in vitro by cloning of the terminator structure in either orientation in vector systems used to study regulatory signals. Termination of transcription at this site was very efficient, both in vivo and in vitro, and in both orientations.

MeSH Terms
Chromosome Mapping Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genes, Regulator Histidine/genetics Operon RNA, Messenger/biosynthesis Rho Factor/genetics Salmonella typhimurium/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Messenger Rho Factor Transcription Factors Histidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carlomagno M S
Riccio A
Bruni C B
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.1.362-368.1985
Published
1985-07-00
Pages
362-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219122
Subset
IM
Databases
GENBANK
M11418
External Links
PubMed source DOI: 10.1128/jb.163.1.362-368.1985 PMC Full Text View journal details
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