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PMID: 3891731 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Enzymatic lysis of the pseudomurein-containing methanogen Methanobacterium formicicum.

Journal of bacteriology ·Vol. 163 ·No. 1 ·1985-07-00 ·Pages 27-36

Bush JW

Abstract

A streptomycete isolated from cow manure produces an extracellular enzyme capable of lysing the pseudomurein-containing methanogen Methanobacterium formicicum. The lytic activity has been partially purified from culture fluid and appears to be a serine protease. Similar lytic activity has been fractionated from pronase. Optimal conditions have been developed for lysis of M. formicicum by commercial preparations of proteinase K. The three lytic enzymes have been partially characterized. The results with the three enzyme preparations tend to confirm that proteolytic enzymes are capable of lysing methanogen cells.

MeSH Terms
Cell Fractionation/methods Cell Wall/metabolism Endopeptidase K Endopeptidases/metabolism Euryarchaeota/metabolism,ultrastructure Kinetics Microscopy, Electron Peptidoglycan/metabolism Pronase/analysis Streptomyces/enzymology Substrate Specificity
Chemicals
Peptidoglycan Endopeptidases Endopeptidase K Pronase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bush J W
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.1.27-36.1985
Published
1985-07-00
Pages
27-36
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219076
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.163.1.27-36.1985 PMC Full Text View journal details
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