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PMID: 3889838 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mac promoters: functional hybrid promoters activated by the malT product and repressed by the lacI product.

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1163-72

Vidal-Ingigliardi D, Raibaud O

Abstract

Using in vitro techniques we have fused upstream sequences from the malPp promoter (normally activated by the MalT protein) to downstream sequences from the lacZp promoter (normally repressed by the LacI protein). Several hybrid promoters were thus obtained, which were controlled by the MalT protein, but were poorly active. More efficient promoters were then isolated using in vivo selection. Three main conclusions could be derived from the analysis of all of these hybrid promoters. Firstly, the MalT protein seems able to force RNA polymerase to start transcription at any DNA sequence, albeit with a low efficiency. Secondly, the strength of the hybrid promoters is considerably increased if a Pribnow Box is positioned at a precise location with respect to the MalT binding site. Thirdly, the presence of the lac operator, even when properly positioned with respect to the transcription startpoint, does not suffice to permit full repression by the lacI product.

MeSH Terms
Base Sequence DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics Genes, Bacterial Genes, Regulator Lac Operon Maltose/genetics Operon Promoter Regions, Genetic Repressor Proteins/genetics Transcription Factors/genetics
Chemicals
DNA, Bacterial DNA, Recombinant Repressor Proteins Transcription Factors Maltose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vidal-Ingigliardi D
Raibaud O
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1163
Published
1985-02-25
Pages
1163-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341063
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.4.1163 PMC Full Text View journal details
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