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PMID: 3888844 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cross-reacting human and rabbit antibodies to antigens of Histoplasma capsulatum, Candida albicans, and Saccharomyces cerevisiae.

Infection and immunity ·Vol. 48 ·No. 3 ·1985-06-00 ·Pages 806-12

Kumar BV, Medoff G, Kobayashi GS, Sieling WL

Abstract

Using Western blots of electrophoretically separated antigens, we show that human antibodies react most frequently to antigens shared by three fungi (Histoplasma capsulatum, Candida albicans, and Saccharomyces cerevisiae). Reactivity to antigens specific for individual fungi was relatively uncommon. The pattern of reactivity could not distinguish infected patients from uninfected controls. Rabbits immunized with extracts of each fungus also produced antibodies to cross-reactive or shared antigens of the other two fungi. Furthermore, preimmune sera showed similar but lower reactivity with the same fungal antigens. We believe that the preimmunization antibodies, which probably resulted from earlier fungal colonization or inapparent infections, predisposed the immune responses elicited by the vaccinations. A similar mechanism likely explains the results with human sera.

MeSH Terms
Animals Antibodies, Fungal/immunology Antigens, Fungal/immunology Candida albicans/immunology Cross Reactions Electrophoresis, Polyacrylamide Gel Histoplasma/immunology Humans Immunization Molecular Weight Rabbits/immunology Saccharomyces cerevisiae/immunology
Chemicals
Antibodies, Fungal Antigens, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kumar B V
Medoff G
Kobayashi G S
Sieling W L
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30 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.48.3.806-812.1985
Published
1985-06-00
Pages
806-12
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261270
Subset
IM
Grants
NIAID NIH HHS · AI 07015 · United States
NIAID NIH HHS · AI 07172 · United States
NIAID NIH HHS · AI 16228 · United States
External Links
PubMed source DOI: 10.1128/iai.48.3.806-812.1985 PMC Full Text View journal details
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