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PMID: 3884199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting.

Clinical and experimental immunology ·Vol. 59 ·No. 2 ·1985-02-00 ·Pages 457-66

Habets WJ, de Rooij DJ, Hoet MH, van de Putte LB, van Venrooij WJ

Abstract

A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibodies were quantitated by an enzyme linked second antibody procedure. Besides being more discriminative, QIBA appeared to be up to 500 times more sensitive than immunodiffusion or immunoelectrophoresis. In this study we used 21 sera from patients with SLE or MCTD for a quantitative analysis of their specific autoantibody content. Within this group, a very diverse spectrum of antibody populations was observed; anti-RNP sera appeared to contain, among others, high tired antibody versus 70K and 31K polypeptides while all (n = 6) anti-Sm sera recognized a 25kD protein doublet. In a follow-up study of two MCTD patients significant flares in specific antibody content could be observed.

MeSH Terms
Adult Aged Antibodies, Antinuclear/analysis Antibody Specificity Antigens/immunology Autoantigens Collodion Dose-Response Relationship, Immunologic Female Follow-Up Studies Humans Immunologic Techniques Lupus Erythematosus, Systemic/immunology Mixed Connective Tissue Disease/immunology Ribonucleoproteins/immunology Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Chemicals
Antibodies, Antinuclear Antigens Autoantigens Ribonucleoproteins Ribonucleoproteins, Small Nuclear snRNP Core Proteins Collodion
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Habets W J
de Rooij D J
Hoet M H
van de Putte L B
van Venrooij W J
References (22)
22 references, click to expand
  1. Polypeptide components of human small nuclear ribonucleoproteins.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5208-12 PMID: 6225117
  2. Antibodies to Sm and RNP. Prognosticators of disease involvement.
    Arthritis Rheum. 1983 Jul;26(7):848-53 PMID: 6603221
  3. Synthesis and assembly of human small nuclear ribonucleoproteins generated by cell-free translation.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6356-60 PMID: 6226047
  4. Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.
    EMBO J. 1983;2(10):1625-31 PMID: 6357777
  5. Identification of human Sm and (U1) RNP antigens by immunoblotting.
    J Immunol Methods. 1983 Nov 11;64(1-2):45-59 PMID: 6227671
  6. Antibodies from patients with mixed connective tissue disease react with heterogeneous nuclear ribonucleoprotein or ribonucleic acid (hnRNP/RNA) of the nuclear matrix.
    J Immunol. 1984 Mar;132(3):1216-22 PMID: 6198384
  7. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA).
    Am J Med. 1972 Feb;52(2):148-59 PMID: 4621694
  8. Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis.
    Arthritis Rheum. 1976 May-Jun;19(3):574-80 PMID: 1084750
  9. Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases.
    N Engl J Med. 1976 Nov 18;295(21):1149-54 PMID: 1086429
  10. Identification of a nuclear protein (Scl-70) as a unique target of human antinuclear antibodies in scleroderma.
    J Biol Chem. 1979 Oct 25;254(20):10514-22 PMID: 385602
  11. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
  12. hnRNA and its attachment to a nuclear protein matrix.
    J Cell Biol. 1981 Mar;88(3):554-63 PMID: 7217204
  13. Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.
    J Immunol. 1982 Mar;128(3):1176-80 PMID: 6173424
  14. Assays for the Sm and RNP autoantigens: the requirement for RNA and influence of the tissue source.
    J Immunol. 1982 Jun;128(6):2751-6 PMID: 6176651
  15. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine.
    Adv Immunol. 1982;33:167-240 PMID: 6182766
  16. Anti-nuclear matrix antibodies in mixed connective tissue disease.
    Eur J Immunol. 1982 Sep;12(9):783-6 PMID: 6183128
  17. Relationship between snRNA species contained in nuclear antigens recognized by autoantibodies and the clinical profile in systemic rheumatic diseases.
    Biomed Pharmacother. 1982;36(3):134-9 PMID: 6185159
  18. Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5, and U6 RNAs.
    J Biol Chem. 1983 Feb 25;258(4):2604-13 PMID: 6185498
  19. 5'-terminal caps of snRNAs are accessible for reaction with 2,2,7-trimethylguanosine-specific antibody in intact snRNPs.
    J Biol Chem. 1983 Mar 10;258(5):2745-7 PMID: 6186660
  20. Clinical characteristics of patients with rheumatic disorders who possess antibodies against ribonucleoprotein particles.
    Arthritis Rheum. 1983 Apr;26(4):509-15 PMID: 6601484
  21. Fractionation and characterization of human small nuclear ribonucleoproteins containing U1 and U2 RNAs.
    J Biol Chem. 1983 Jun 10;258(11):7181-9 PMID: 6189835
  22. Antibodies against distinct nuclear matrix proteins are characteristic for mixed connective tissue disease.
    Clin Exp Immunol. 1983 Oct;54(1):265-76 PMID: 6352106
Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1985-02-00
Pages
457-66
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1577150
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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