Molecular cloning and expression of the xylanase gene of alkalophilic Bacillus sp. strain C-125 in Escherichia coli.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 784-5

Honda H, Kudo T, Horikoshi K

Abstract

The gene for xylanase A of alkalophilic Bacillus sp. strain C-125 was cloned in Escherichia coli with pBR322. The plasmid pCX311 contained 2.6- and 2.0-kilobase-pair HindIII fragments. The characteristics of the purified pCX311-encoded xylanase were the same as those of purified xylanase A from alkalophilic Bacillus sp. strain C-125.

MeSH Terms

Bacillus/genetics Cloning, Molecular Escherichia coli/genetics Glycoside Hydrolases/genetics Plasmids Xylan Endo-1,3-beta-Xylosidase

Chemicals

Glycoside Hydrolases Xylan Endo-1,3-beta-Xylosidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Honda H

Kudo T

Horikoshi K

References (3)

3 references, click to expand

Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
A rapid boiling method for the preparation of bacterial plasmids.
Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.161.2.784-785.1985

Published

1985-02-00

Pages

784-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214955

Subset

External Links

PubMed source DOI: 10.1128/jb.161.2.784-785.1985 PMC Full Text View journal details

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Molecular cloning and expression of the xylanase gene of alkalophilic Bacillus sp. strain C-125 in Escherichia coli.

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