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PMID: 3865187 Published · ppublish English Journal Article

Amplification of DNA sequences in human multidrug-resistant KB carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 22 ·1985-11-00 ·Pages 7661-5

Fojo AT, Whang-Peng J, Gottesman MM, Pastan I

Abstract

Four KB carcinoma cell lines selected independently for resistance to either colchicine, adriamycin, or vinblastine were studied. All cell lines showed high levels of resistance to the selecting drug and cross-resistance to the other drugs and to actinomycin D. Double-minute chromosomes could be identified on chromosomal spreads of these multidrug-resistant KB cell lines. Amplification of specific DNA sequences was demonstrated by using the technique of in-gel renaturation. All the cell lines share common amplified sequences. There are also amplified sequences that are specific for each cell line. A revertant cell line that has reacquired drug sensitivity has lost its amplified sequences. Specific probes obtained by cloning amplified sequences from the cell line selected in vinblastine recognize amplified sequences in all the resistant lines. The presence of common amplified sequences in these cell lines is strong evidence for the importance of these regions in multiple drug resistance.

MeSH Terms
Base Sequence Colchicine/pharmacology DNA, Neoplasm/analysis Doxorubicin/pharmacology Drug Resistance Gene Amplification Humans KB Cells/drug effects Phenotype Vinblastine/pharmacology
Chemicals
DNA, Neoplasm Vinblastine Doxorubicin Colchicine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fojo A T
Whang-Peng J
Gottesman M M
Pastan I
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.22.7661
Published
1985-11-00
Pages
7661-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391393
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.22.7661 PMC Full Text View journal details
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