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PMID: 3865175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphorylation of the yeast equivalent of ribosomal protein S6 is not essential for growth.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 22 ·1985-11-00 ·Pages 7515-9

Kruse C, Johnson SP, Warner JR

Abstract

The yeast equivalent of ribosomal protein S6, known as S10, can be modified by the addition of two phosphates. The two adjacent serines that are likely to be subject to phosphorylation were deduced by comparison with the known sites of phosphorylation on rat liver S6. Using oligonucleotide mutagenesis, we altered the gene for S10 to replace these two serines with alanines. This mutant gene was introduced into a diploid yeast cell heterozygous for each of the two S10 genes. After sporulation, we obtained colonies in which the mutant gene was the only intact S10 gene. Although the ribosomes of these cells contained a full complement of S10, no phosphorylation of S10 was detected. These cells grow exponentially with a doubling time about 50% greater than that of control cells. We conclude that the phosphorylation of S10 is not essential for growth. However, the mutant gene in such cells is very unstable, frequently reverting to wild type, presumably by interaction with the disrupted host genes. We suggest that at some stage of the growth cycle there is strong selection for S10 that can be phosphorylated.

MeSH Terms
Fungal Proteins/metabolism Mutation Phosphorylation Ribosomal Proteins/genetics,metabolism Yeasts/growth & development,metabolism
Chemicals
Fungal Proteins Ribosomal Proteins ribosomal protein S10
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kruse C
Johnson S P
Warner J R
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.22.7515
Published
1985-11-00
Pages
7515-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390847
Subset
IM
Grants
NCI NIH HHS · 3PO CA13330 · United States
NIGMS NIH HHS · 5RO1 GM25532 · United States
NCI NIH HHS · 5T32 CA09060 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.22.7515 PMC Full Text View journal details
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