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PMID: 3855548 Published · ppublish English Journal Article

Reovirus hemagglutinin mRNA codes for two polypeptides in overlapping reading frames.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 1 ·1985-01-00 ·Pages 48-52

Ernst H, Shatkin AJ

Abstract

Human reovirus s1 mRNA, which codes for the viral hemagglutinin, also directs the synthesis of a previously unrecognized polypeptide of molecular mass 14 kDa in reticulocyte and wheat germ extracts. Hybrid-arrest of translation by selected restriction fragments of cloned S1 DNA indicated that synthesis of the 14-kDa polypeptide initiates at the second AUG. This was confirmed by NH2-terminal sequence analyses. The coding sequence for the 14-kDa polypeptide thus lies entirely within the hemagglutinin gene but in a different reading frame. Although not found in virions, the 14-kDa polypeptide apparently is formed in virus-infected mouse L cells, as demonstrated by comparison of [35S]methionine-labeled polypeptides in cell extracts with the corresponding in vitro products.

MeSH Terms
Amino Acid Sequence Cell-Free System Hemagglutinins, Viral/genetics Mammalian orthoreovirus 3/genetics Peptide Fragments/analysis Protein Biosynthesis RNA, Double-Stranded/genetics RNA, Messenger/genetics RNA, Viral/genetics Reoviridae/genetics Viral Proteins/genetics
Chemicals
Hemagglutinins, Viral Peptide Fragments RNA, Double-Stranded RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ernst H
Shatkin A J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.1.48
Published
1985-01-00
Pages
48-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC396968
Subset
IM
Databases
GENBANK
J02325
External Links
PubMed source DOI: 10.1073/pnas.82.1.48 PMC Full Text View journal details
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