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PMID: 3844977 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of an anti-CEA monoclonal antibody in colo-rectal carcinoma xenografts.

Cancer immunology, immunotherapy : CII ·Vol. 19 ·No. 1 ·1985-00-00 ·Pages 8-17

Pimm MV, Armitage NC, Perkins AC, Smith W, Baldwin RW

Abstract

A mouse monoclonal anti-CEA antibody (11.285.14) has been examined for tumour localization potential by assessing its distribution in immunodeprived mice with xenografts of human colon carcinoma cell lines HCT-8, HRT-18, HT-29, and LS174T and a xenograft (HRVB) established from a primary rectal carcinoma. With four carcinomas (HCT-8, HT-29, LS174T, and HRVB) preferential tumour localization of 125I anti-CEA was seen. Compared with 131I normal IgG1 localization indices of up to 4.4:1 were achieved. Up to 10% of the injected dose of 125I anti-CEA was present/g of tumour tissue and with the largest xenografts examined (3-4 g) up to 40% of the total body reactivity was localized in tumour tissue. The tumour localization of 131I labelled antibody was visualized by external gamma camera imaging. Overall antibody localization correlated with the CEA content of the xenografts and the fourth colon carcinoma xenograft (HRT-18) and an osteogenic sarcoma xenograft (791T), both with very low CEA levels, showed no localization of the monoclonal antibody.

MeSH Terms
Animals Antibodies, Monoclonal Autoradiography Carcinoembryonic Antigen/analysis Carcinoma/immunology Colonic Neoplasms/immunology Humans Mice Mice, Inbred CBA Neoplasm Transplantation Neoplasms, Experimental/diagnosis,diagnostic imaging Radionuclide Imaging Rectal Neoplasms/immunology
Chemicals
Antibodies, Monoclonal Carcinoembryonic Antigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pimm M V
Armitage N C
Perkins A C
Smith W
Baldwin R W
References (18)
18 references, click to expand
  1. Binding of five monoclonal anti-CEA antibodies with different epitope specificities to various carcinoma tissues.
    Int J Cancer. 1984 Apr 15;33(4):469-75 PMID: 6200447
  2. In vivo localization of anti-osteogenic sarcoma 791T monoclonal antibody in osteogenic sarcoma xenografts.
    Int J Cancer. 1982 Jul 15;30(1):75-85 PMID: 6956558
  3. Improved immune-suppression techniques for the exongrafting of human tumours.
    Br J Cancer. 1978 Feb;37(2):224-30 PMID: 343803
  4. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy.
    J Clin Pathol. 1982 Nov;35(11):1253-67 PMID: 6183295
  5. Experimental studies of tumor radioimmunodetection using antibody mixtures against carcinoembryonic antigen (CEA) and colon-specific antigen-p (CSAp).
    Int J Cancer. 1981 Jan 15;27(1):101-5 PMID: 7251228
  6. Localization of human tumour xenografts after i.v. administration of radiolabeled monoclonal antibodies.
    Br J Cancer. 1981 Jul;44(1):91-9 PMID: 6789857
  7. Tumor localization of CEA-containing human tumors in nude mice by means of monoclonal anti-CEA antibodies.
    Int J Cancer. 1982 Nov 15;30(5):547-52 PMID: 7152719
  8. Imaging of human melanoma xenografts in nude mice with a radiolabeled monoclonal antibody.
    J Natl Cancer Inst. 1982 Oct;69(4):823-6 PMID: 6956760
  9. Imaging of melanoma with L-131-labeled monoclonal antibodies.
    J Nucl Med. 1983 Feb;24(2):123-9 PMID: 6822875
  10. Immunological heterogeneity of carcinoembryonic antigen: antigenic determinants on carcinoembryonic antigen distinguished by monoclonal antibodies.
    Cancer Res. 1983 Feb;43(2):686-92 PMID: 6184152
  11. Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintigraphy.
    Immunol Today. 1981 Dec;2(12):239-49 PMID: 25290651
  12. Specificities and binding properties of eight monoclonal antibodies against carcinoembryonic antigen.
    Mol Immunol. 1982 Dec;19(12):1641-8 PMID: 6186908
  13. Tumour localisation with monoclonal antibody radioactivity labelled with metal chelate rather than iodine.
    Lancet. 1982 Dec 11;2(8311):1347-8 PMID: 6128634
  14. Tomoscintigraphy for detecting gastrointestinal and medullary thyroid cancers: first clinical results using radiolabelled monoclonal antibodies against carcinoembryonic antigen.
    Br Med J (Clin Res Ed). 1982 Nov 20;285(6353):1447-51 PMID: 6814596
  15. Radiolabeled fragments of monoclonal antibodies against carcinoembryonic antigen for localization of human colon carcinoma grafted into nude mice.
    J Exp Med. 1983 Aug 1;158(2):413-27 PMID: 6886623
  16. Localization of I-131-labeled tumor-specific monoclonal antibody in the tumor-bearing BALB/c mouse.
    J Nucl Med. 1980 Jun;21(6):570-3 PMID: 7381576
  17. Quantitative evaluation of the localization of a monoclonal antibody (791T/36) in human osteogenic sarcoma xenografts.
    Eur J Cancer Clin Oncol. 1984 Apr;20(4):515-24 PMID: 6586422
  18. Monoclonal antibodies to carcinoembryonic antigen: ionic strength as a factor in the selection of antibodies for immunoscintigraphy.
    Cancer Res. 1983 Aug;43(8):3857-64 PMID: 6344991
Article Info
Journal
Cancer immunology, immunotherapy : CII
Abbr.
Cancer Immunol Immunother
ISSN
0340-7004
DOI
10.1007/BF00199305
Published
1985-00-00
Pages
8-17
Language
English
Region
Germany
NLM ID
8605732
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00199305 View journal details
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