Acylation of adenovirus type 12 early region 1b 18-kDa protein. Further evidence for its localisation in the cell membrane.

The 18-kDa E1b protein in Ad 12-transformed rat cells and in Ad 12-infected human cells binds lipid strongly. The lipid is not removed by boiling in the presence of SDS or by extraction with methanol/chloroform. It is, however, dissociated from the protein by treatment with methanolic KOH suggesting that attachment is through an ester linkage. The acylated 18-kDa protein is detected only in the membrane fraction. Labelling cell surface proteins on Ad 12-transformed cells with [125I]iodosulphanilic acid shows that some of the Ad 12 18-kDa E1b protein is present on the outside of the cell. It is concluded that this protein is responsible for cell surface T-antigen activity.