Assembly of ribosomal subunits affected in a ribosomal mutant of E. coli having an altered L22 protein.

Molecular & general genetics : MGG ·Vol. 174 ·No. 1 ·1979-07-02 ·Pages 53-8

Pardo D, Vola C, Rosset R

Abstract

In this article we describe some in vivo properties of a coldsensitive ribosomal mutant from Escherichia coli. The mutation affects the rplV gene which is the structural gene of ribosomal protein L22. Our work shows that at 22 degrees C, the biosynthesis of both ribosomal subunits and the maturation processing of 15S and 23S ribosomal RNA are impaired. Integration of our results in a general model of in vivo ribosomal assembly in E. coli is presented.

MeSH Terms

Cold Temperature Escherichia coli/genetics Genes Mutation Phenotype RNA, Ribosomal/biosynthesis Ribosomal Proteins/biosynthesis,genetics

Chemicals

RNA, Ribosomal Ribosomal Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pardo D

Vola C

Rosset R

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00433305

Published

1979-07-02

Pages

53-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00433305

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Assembly of ribosomal subunits affected in a ribosomal mutant of E. coli having an altered L22 protein.

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