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PMID: 3838595 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Insertion mutagenesis of embryonal carcinoma cells by retroviruses.

Science (New York, N.Y.) ·Vol. 228 ·No. 4699 ·1985-05-03 ·Pages 554-8

King W, Patel MD, Lobel LI, Goff SP, Nguyen-Huu MC

Abstract

Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.

MeSH Terms

Animals Base Sequence Cell Line DNA/genetics DNA, Neoplasm/genetics DNA, Recombinant/metabolism DNA, Viral/genetics Mice Moloney murine leukemia virus/physiology Mutation Nucleic Acid Hybridization Rats Retroviridae/physiology Teratoma/genetics,microbiology

Chemicals

DNA, Neoplasm DNA, Recombinant DNA, Viral DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

King W

Patel M D

Lobel L I

Goff S P

Nguyen-Huu M C

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.3838595

Published

1985-05-03

Pages

554-8

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.3838595 View journal details

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