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PMID: 3828656 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

P2-purinoceptors of two subtypes in the rabbit mesenteric artery: reactive blue 2 selectively inhibits responses mediated via the P2y-but not the P2x-purinoceptor.

British journal of pharmacology ·Vol. 90 ·No. 2 ·1987-02-00 ·Pages 383-91

Burnstock G, Warland JJ

Abstract

alpha,beta-Methylene ATP and ATP both produced concentration-dependent contractions of the isolated mesenteric artery of the rabbit that were not inhibited by reactive blue 2. In preparations where the tone had been raised with noradrenaline, ATP and 2-methylthio ATP, but not alpha,beta-methylene ATP, produced relaxations of the vessel. These relaxations were inhibited in the presence of reactive blue 2. Reactive blue 2 did not inhibit the contractions to noradrenaline, and only slightly inhibited relaxations to adenosine and acetylcholine. The rank order of potency of purine nucleotide analogues in contracting the vessel was: alpha,beta-methylene ATP greater than beta,gamma-methylene ATP = 2-methylthio ATP greater than ATP, and in relaxing the vessel at raised tone was: 2-methylthio ATP greater than ATP greater than beta,gamma-methylene ATP greater than alpha,beta-methylene ATP. It is concluded from this study that in the isolated mesenteric artery of the rabbit, purine nucleotides act via P2y-purinoceptors to cause the muscle to relax and via P2x-purinoceptors to cause the muscle to contract. The results also suggest that reactive blue 2 selectively inhibits responses mediated via the P2y-purinoceptor, at least within a limited concentration range.

MeSH Terms

Adenosine Triphosphate/analogs & derivatives,antagonists & inhibitors,pharmacology Animals In Vitro Techniques Male Mesenteric Arteries/drug effects Rabbits Receptors, Purinergic/classification,drug effects Triazines/pharmacology Vasoconstriction/drug effects

Chemicals

Receptors, Purinergic Triazines Cibacron Blue F 3GA Adenosine Triphosphate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Burnstock G

Warland J J

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30 references, click to expand

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Article Info

Journal

British journal of pharmacology

Abbr.

Br J Pharmacol

ISSN

0007-1188

DOI

10.1111/j.1476-5381.1987.tb08968.x

Published

1987-02-00

Pages

383-91

Language

English

Region

England

NLM ID

7502536

PMCID

PMC1916939

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1987.tb08968.x PMC Full Text View journal details

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P2-purinoceptors of two subtypes in the rabbit mesenteric artery: reactive blue 2 selectively inhibits responses mediated via the P2y-but not the P2x-purinoceptor.

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