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PMID: 38260 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cross-linking of fibronectin to collagen by blood coagulation Factor XIIIa.

The Journal of clinical investigation ·Vol. 64 ·No. 3 ·1979-09-00 ·Pages 781-7

Mosher DF, Schad PE

Abstract

Soluble fibronectin is found in body fluids and media of adherent cultured cells and binds to fibrin and collagen. Insoluble fibronectin is found in tissue stroma and in extracellular matrices of cultured cells. Fibronectin is a substrate for Factor XIIIa (plasma transglutaminase) and can be cross-linked by Factor XIIIa to itself and the the alpha-chain of fibrin. We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis to investigate Factor XIIIa-mediated crosslinking of fibronectin to collagen. At O degrees or 37 degrees C, fibronectin could be cross-linked to iodinated cyanogen bromide fragment 7 of the alpha 1(I) chain. At 22 degrees or 37 degrees C, fibronectin could be cross-linked to isolated alpha 1(I) chains of type I collagen. Fibronectin could also be crosslinked to types I and III collagen, but only at 37 degrees C. alpha 1(I)-CB7, alpha 1(I) collagen chains, type I collagen, type III collagen, and fibrin all blocked cross-linking between 125I-alpha 1 (I)-CB7 and fibronectin. alpha 1(I)-CB7 blocked cross-linking between fibronectin and fibrin. These results indicate that the determinants of fibronectin-fibrin and fibronectin-collagen binding and cross-linking are similar. Cross-linking of fibronectin to collagen likely occurs in vivo and may be important for normal wound healing, collagen fibrillogenesis, and embryogenesis.

MeSH Terms
Binding Sites Collagen/metabolism Fibrin/pharmacology Glycoproteins/blood,metabolism In Vitro Techniques Molecular Conformation Molecular Weight Wound Healing gamma-Glutamyltransferase/metabolism
Chemicals
Glycoproteins Fibrin Collagen gamma-Glutamyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mosher D F
Schad P E
References (44)
44 references, click to expand
  1. Polymerization of a major surface-associated glycoprotein, fibronectin, in cultured fibroblasts.
    FEBS Lett. 1976 Dec 1;71(2):325-9 PMID: 1033841
  2. Induction of human fibroblast proliferation by epidermal growth factor (EGF): enhancement by an EGF-binding arginine esterase and by ascorbate.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):183-7 PMID: 1061114
  3. Cross-linking of cold-insoluble globulin by fibrin-stabilizing factor.
    J Biol Chem. 1975 Aug 25;250(16):6614-21 PMID: 1158872
  4. Proceedings: Factor XIII, fibrin and collagen.
    Thromb Diath Haemorrh. 1975 Nov 15;34(2):551 PMID: 1198420
  5. Fibrin stabilizing factor (F XIII) and collagen polymerization.
    Experientia. 1975 Nov 15;31(11):1355-7 PMID: 1239386
  6. The resolution of Ascaris cuticle collagen into three chain types.
    Biochemistry. 1976 Apr 6;15(7):1435-9 PMID: 1259947
  7. Recongnition by guinea-pig peritoneal exudate cells of conformationally different states of the collagen molecule.
    Immunology. 1976 Feb;30(2):249-59 PMID: 1262053
  8. Cold-insoluble globulin, II[1,2]. Cyanogen bromide and plasminolysis fragments containing a label introduced by transamidation.
    Hoppe Seylers Z Physiol Chem. 1977 Sep;358(9):1165-8 PMID: 144694
  9. Bovine plasma cold-insoluble globulin: gross structure and function.
    Ann N Y Acad Sci. 1978 Jun 20;312:56-73 PMID: 159011
  10. Human thrombins. Production, evaluation, and properties of alpha-thrombin.
    J Biol Chem. 1977 Jun 10;252(11):3587-98 PMID: 16908
  11. Localization of the binding site for cell attachment in the alpha1(I) chain of collagen.
    J Biol Chem. 1978 Aug 25;253(16):5642-6 PMID: 209046
  12. Codistribution of pericellular matrix proteins in cultured fibroblasts and loss in transformation: fibronectin and procollagen.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4944-8 PMID: 217006
  13. Binding of cell attachment protein to collagen: effect of chemical modifications.
    Ann N Y Acad Sci. 1978 Jun 20;312:436-8 PMID: 291378
  14. Distribution of cold-insoluble globulin in plasma and tissues.
    Ann N Y Acad Sci. 1978 Jun 20;312:74-92 PMID: 291382
  15. Cell surface-associated structural proteins in connective tissue cells.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2480-4 PMID: 329287
  16. Immunocytochemical localization of fibronectin (LETS proteins) on the surface of L6 myoblasts: light and electron microscopic studies.
    Cell. 1978 Feb;13(2):263-71 PMID: 342116
  17. External fibronectin of cultured human fibroblasts is predominantly a matrix protein.
    J Cell Biol. 1978 Mar;76(3):748-60 PMID: 344328
  18. Fibronectins--adhesive glycoproteins of cell surface and blood.
    Nature. 1978 Sep 21;275(5677):179-84 PMID: 357987
  19. High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation.
    Biochim Biophys Acta. 1978 Sep 18;516(1):1-25 PMID: 361081
  20. Cellular transglutaminase, growth, and transformation.
    Ann N Y Acad Sci. 1978 Jun 20;312:354-65 PMID: 40517
  21. The cold-insoluble globulin of human plasma. I. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components.
    J Biol Chem. 1970 Nov 10;245(21):5728-36 PMID: 4097343
  22. The chemistry and structure of collagen.
    Adv Protein Chem. 1971;25:243-352 PMID: 4111984
  23. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
    J Biol Chem. 1974 Jan 25;249(2):634-44 PMID: 4129205
  24. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  25. Documentation of the plasma factor XIII deficiency in man.
    Ann N Y Acad Sci. 1972 Dec 8;202:190-9 PMID: 4508922
  26. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
    Biochem J. 1973 Jul;133(3):529-39 PMID: 4733239
  27. Action of fibrin-stabilizing factor on cold-insoluble globulin and alpha2-macroglobulin in clotting plasma.
    J Biol Chem. 1976 Mar 25;251(6):1639-45 PMID: 56335
  28. Affinity of fibronectin to collagens of different genetic types and to fibrinogen.
    J Exp Med. 1978 Jun 1;147(6):1584-95 PMID: 567240
  29. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  30. Identification of the sites in collagen alpha-chains that bind serum anti-gelatin factor (cold-insoluble globulin).
    Biochem J. 1978 Jan 1;169(1):55-9 PMID: 629753
  31. Similarity of antigelatin factor and cold insoluble globulin.
    Biochim Biophys Acta. 1978 Mar 28;533(1):227-37 PMID: 638188
  32. Cold-insoluble globulin (fibronectin), IV[1-35 affinity to soluble collagen of various types.
    Hoppe Seylers Z Physiol Chem. 1978 Feb;359(2):247-50 PMID: 649060
  33. Studies on intercellular LETS glycoprotein matrices.
    Cell. 1978 Jun;14(2):377-91 PMID: 667946
  34. The amino acid sequence of the carboxy-terminal 142 amino acids of the alpha-chain of human fibrinogen.
    Thromb Res. 1978 Jun;12(6):1135-46 PMID: 694821
  35. Amniotic fluid fibronectin. Characterization and synthesis by cells in culture.
    J Cell Biol. 1978 Sep;78(3):701-15 PMID: 701356
  36. Amino acid sequence of human fibrin. Preliminary note on the completion of the C-terminal part of the alpha-chain sequence.
    Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1451-5 PMID: 721075
  37. The epsilon-(gamma-glutamyl)lysine crosslink and the catalytic role of transglutaminases.
    Adv Protein Chem. 1977;31:1-133 PMID: 73346
  38. Thrombin stimulates the production and release of a major surface-associated glycoprotein (fibronectin) in cultures of human fibroblasts.
    Exp Cell Res. 1978 Mar 15;112(2):323-34 PMID: 75807
  39. Metachromasy of peripheral nerve collagen on polyacrylamide gels stained with Coomassie brilliant blue R-250.
    Anal Biochem. 1978 Aug 1;88(2):566-72 PMID: 80963
  40. Labeling of a major fibroblast surface protein (fibronectin) catalyzed by blood coagulation factor XIIa.
    Biochim Biophys Acta. 1977 Mar 28;491(1):205-10 PMID: 849457
  41. Thrombin potentiates the mitogenic response of cultured fibroblasts to serum and other growth promoting agents.
    J Cell Physiol. 1977 Aug;92(2):233-9 PMID: 881434
  42. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.
    Int J Cancer. 1977 Jul 15;20(1):1-5 PMID: 903179
  43. Cross-linking of a major fibroblast surface-associated glycoprotein (fibronectin) catalyzed by blood coagulation factor XIII.
    Cell. 1976 Sep;9(1):29-35 PMID: 975239
  44. Localization of the cell attachment region in types I and II collagens.
    Biochem Biophys Res Commun. 1976 SEP 20;72(2):426-32 PMID: 985489
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI109524
Published
1979-09-00
Pages
781-7
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC372182
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI109524 PMC Full Text View journal details
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