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PMID: 38072997 Published · ppublish English Journal Article

Preliminary study of HPV integration status on the occurrence and development of vaginal intraepithelial neoplasia.

The journal of obstetrics and gynaecology research ·Vol. 50 ·No. 3 ·2024-03-00 ·Pages 478-484

Yuan S, Qian C, Zhang H, Xing Y

Abstract

The aim of this study was to investigate how the integration status of HPV in the vaginal epithelium affects the development of vaginal intraepithelial neoplasia (VaIN). Twenty-four vaginal tissues were collected before applying high-throughput viral integration detection (HIVID), medical records of them were documented, including age, thin-prep cytologic test (TCT) and HPV test results, colposcopic biopsy pathology, and other clinical data, such as history of total hysterectomy for cervical lesions, whether they were infected with HPV16/18 with a follow-up span of 2 years. We summarized the distribution of HPV integration on the host chromosome and HPV type, as well as the hotspot integration gene and its role in the development of VaIN. In this study, 24 cases suffered from VaIN were involved. HPV integration was detected in 11 cases; furthermore, we discovered HPV 16 and 73, chromosome 1 and 2 possessed most HPV integration sites while EMBP1, CLO5A1, EHF, ELF5 as dominate hot spots. Taken clinical outcome into account, we found a significant difference between HPV integration occurrence and VaIN (p = 0.011). (1) This study found a statistical difference between HPV integration and the occurrence of VaIN; (2) HPV integration may provide a new clinical predictor for VaIN and facilitate risk assessment and stratified management of high-risk patients.

Keywords
HPV integration clinical outcome high-throughput viral integration detection integration sites vaginal intraepithelial neoplasm
MeSH Terms
Female Humans Child, Preschool Human papillomavirus 16 Human papillomavirus 18 Papillomavirus Infections/complications Biopsy Neoplasms
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yuan Shuning ORCID
Department of Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Qian Cheng
Department of Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Zhang Hailong
Department of Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Xing Yan ORCID
Department of Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
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26 references, click to expand
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Article Info
Journal
The journal of obstetrics and gynaecology research
Abbr.
J Obstet Gynaecol Res
ISSN
1447-0756
DOI
10.1111/jog.15855
Published
2024-03-00
Epub
2023-00-10
Pages
478-484
Language
English
Region
Australia
NLM ID
9612761
Subset
IM
Grants
Jiangsu Commission of Health (General Program) · No H2019009
External Links
PubMed source DOI: 10.1111/jog.15855 View journal details
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