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PMID: 3800968 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PAF-acether (1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphocholine)-induced fibrinogen binding to platelets depends on metabolic energy.

The Biochemical journal ·Vol. 238 ·No. 3 ·1986-09-15 ·Pages 885-91

Kloprogge E, Hasselaar P, Akkerman JW

Abstract

A combination of CN- and 2-deoxy-D-glucose decreases the binding of fibrinogen to platelets stimulated with PAF-acether (1-O-hexadecyl/octadecyl-2-acetyl-sn-glycero-3-phosphocholine). Decreased binding is found after pretreatment with metabolic inhibitors, thereby lowering the energy content before stimulation as well as at various stages after stimulation of undisturbed cells. Binding and ATP hydrolysis occur in parallel, suggesting tight coupling between both phenomena. Energy appears to be predominantly required for exposure and maintenance of accessible binding sites, whereas the interaction between fibrinogen and the exposed sites does not depend on metabolic energy.

MeSH Terms
Blood Platelets/drug effects,metabolism Cyanides/pharmacology Deoxyglucose/pharmacology Energy Metabolism/drug effects Fibrinogen/metabolism Humans Platelet Activating Factor/analogs & derivatives,pharmacology Platelet Aggregation/drug effects Protein Binding/drug effects Stimulation, Chemical
Chemicals
Cyanides Platelet Activating Factor 1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine Fibrinogen Deoxyglucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kloprogge E
Hasselaar P
Akkerman J W
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28 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2380885
Published
1986-09-15
Pages
885-91
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147218
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2380885 PMC Full Text View journal details
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