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PMID: 3790076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intralipid administration induces a lipoprotein lipase-like activity in the livers of starved adult rats.

The Biochemical journal ·Vol. 236 ·No. 1 ·1986-05-15 ·Pages 273-8

Vilaró S, Reina M, Ramírez I, Llobera M

Abstract

The administration of Intralipid to starved adult rats induces the appearance of lipoprotein lipase (LPL)-like activity in the liver, whereas the so-called hepatic triacylglycerol lipase is unaffected. This LPL-like activity is eluted by 1.5 M-NaCl from heparin-Sepharose columns. This partially purified fraction is inhibited by 1.0 M-NaCl (91%) and by 1.0 mg of protamine sulphate/ml (79%), whereas it is stimulated 69-fold by the presence of 8.0 micrograms of apolipoprotein C-II/ml and inhibited by anti-LPL antibodies. We conclude that Intralipid administration induces the appearance of LPL activity in livers of starved adult rats. Its possible origin is discussed.

MeSH Terms
Animals Antibodies/immunology Apolipoproteins/pharmacology Chromatography, Affinity Fat Emulsions, Intravenous/pharmacology Lipolysis Lipoprotein Lipase/immunology,metabolism Liver/enzymology Male Protamines/pharmacology Rats Rats, Inbred Strains Sodium Chloride/pharmacology Starvation/enzymology Triglycerides/blood
Chemicals
Antibodies Apolipoproteins Fat Emulsions, Intravenous Protamines Triglycerides Sodium Chloride Lipoprotein Lipase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vilaró S
Reina M
Ramírez I
Llobera M
References (35)
35 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2360273
Published
1986-05-15
Pages
273-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1146816
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2360273 PMC Full Text View journal details
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