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PMID: 378980 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

Journal of bacteriology ·Vol. 139 ·No. 2 ·1979-08-00 ·Pages 608-19

Hashimoto-Gotoh T, Inselburg J

Abstract

Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid incompatibility lies between base pairs -185 and -684; (ii) the integrity of at least part of the region of ColE1 DNA between base pairs -185 and -572 is essential for the expression of ColE1 incompatibility; (iii) the expression of incompatibility is independent of the ability of the ColE1 genome to replicate autonomously; (iv) plasmid incompatibility is affected by plasmid copy number; and (v) ColE1 plasmid-mediated DNA replication of the lambda phage-ColE1 chimera lambda imm434 Oam29 Pam3 ColE1 is inhibited by ColE1-incompatible but not by ColE1-compatible plasmids.

MeSH Terms
Colicins DNA Replication DNA Restriction Enzymes DNA, Recombinant Escherichia coli/genetics Genes Mutation Plasmids
Chemicals
Colicins DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hashimoto-Gotoh T
Inselburg J
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.2.608-619.1979
Published
1979-08-00
Pages
608-19
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216910
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.2.608-619.1979 PMC Full Text View journal details
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