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PMID: 378383 Published · ppublish English Journal Article

Immunocytochemical localization of a calcium-binding protein in the rat duodenum.

Cell and tissue research ·Vol. 197 ·No. 1 ·1979-03-09 ·Pages 69-77

Marche P, Le Guern C, Cassier P

Abstract

The cellular localization of the vitamin D-dependent calcium-binding protein (CaBP) in the duodenum of rat was studied using indirect immunofluorescence and immunoperoxidase-staining methods. Specific positive reaction product, indicative of the presence of CaBP, was exclusively located within the villous part of the duodenal mucosa. Moreover, CaBP was detected mainly within the supranuclear region of the cytoplasm of absorptive cells and also at the level of their basal laminae. CaBP was not demonstrable either in the nuclei or associated with the brush border membrane of absorptive cells. Also, CaBP was neither detectable in goblet cells nor in sub-epithelial layers. When the specific anti-CaBP antiserum was replaced by nonimmune rabbit serum or when it was preabsorbed on a CaBP-Sepharose conjugate, no positive immunostaining was seen. Together with recent biochemical data our observations agree well with the view that CaBP may act as an intracellular "buffer" by protecting the cell against too high Ca2+ concentrations.

MeSH Terms
Animals Carrier Proteins/isolation & purification Cytoplasm/analysis Duodenum/analysis Fluorescent Antibody Technique Histocytochemistry Immunoenzyme Techniques Intestinal Mucosa/analysis Microvilli/analysis Rats S100 Calcium Binding Protein G/isolation & purification
Chemicals
Carrier Proteins S100 Calcium Binding Protein G
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Marche P
Le Guern C
Cassier P
References (19)
19 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00233554
Published
1979-03-09
Pages
69-77
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00233554 View journal details
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