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PMID: 3774373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vaccination of mice against malaria with soluble antigens. I. The effect of detergent, route of injection, and adjuvant.

Parasite immunology ·Vol. 8 ·No. 5 ·1986-09-00 ·Pages 409-14

Playfair JH, De Souza JB

Abstract

Mice were vaccinated against lethal blood stage P. yoelii malaria with lysates of parasitised blood prepared in various detergents. Two injections, containing 25 micrograms of protein, of a Triton X-100 lysate gave optimal protection when injected intraperitoneally with saponin as adjuvant. Such lysates provide a starting point for the isolation of potential antigens for vaccination, selected for their protective activity in vivo.

MeSH Terms

Adjuvants, Immunologic/pharmacology Animals Antigens, Protozoan/immunology Detergents/pharmacology Female Injections, Intraperitoneal Injections, Subcutaneous Malaria/prevention & control Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Plasmodium/immunology Saponins/pharmacology Vaccination Vaccines/administration & dosage

Chemicals

Adjuvants, Immunologic Antigens, Protozoan Detergents Saponins Vaccines

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Playfair J H

De Souza J B

Article Info

Journal

Parasite immunology

Abbr.

Parasite Immunol

ISSN

0141-9838

DOI

10.1111/j.1365-3024.1986.tb00857.x

Published

1986-09-00

Pages

409-14

Language

English

Region

England

NLM ID

7910948

Subset

External Links

PubMed source DOI: 10.1111/j.1365-3024.1986.tb00857.x View journal details

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Vaccination of mice against malaria with soluble antigens. I. The effect of detergent, route of injection, and adjuvant.

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