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PMID: 377235 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparison on the structure and transcriptional capability of growing phase and stationary yeast chromatin: a model for reversible gene activation.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 1909-27

Lohr D, Ide G

Abstract

We have compared the structure of intra-nuclear and isolated chromatin from logarithmically growing yeast cells to chromatin from cells which had entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern, 160 bp repeat size, with staphylococcal nuclease and similar variability in repeat sizes within the genome. DNase I produces the same ladder (less than 120 b) and a quite similar extended ladder (120-300 b) which shows that both chromatins have phased nucleosomes. However, the rate of DNase I digestion of growing phase is greater than in stationary. Functionally speaking, growing phase nuclei are 5-20 times as active in the rate of endogenous transcription (all three polymerases are involved). The transcriptional and DNase I susceptibility differences noted in nuclei are maintained in sucrose gradient isolated oligonucleosomes and mononucleosomes from the two states.

MeSH Terms
Amanitins/pharmacology Cell Nucleus/metabolism Chromatin/metabolism,ultrastructure DNA/metabolism Deoxyribonucleases Kinetics Micrococcal Nuclease Potassium/pharmacology RNA/biosynthesis Saccharomyces cerevisiae/growth & development,metabolism Transcription, Genetic/drug effects
Chemicals
Amanitins Chromatin RNA DNA Deoxyribonucleases Micrococcal Nuclease Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lohr D
Ide G
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.5.1909
Published
1979-00-00
Pages
1909-27
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327820
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.5.1909 PMC Full Text View journal details
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