Physical map of the seven ribosomal RNA genes of Escherichia coli.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 1817-30

Boros I, Kiss A, Venetianer P

Abstract

Escherichia coli DNA was digested with restriction endonucleases BamHI, PstI, EcoRI, SalI, HindIII, XhoI, BglII, SmaI, HpaI and with selected double and triple combinations of the same enzymes. The digests were electrophoresed and hybridized with 32P-labelled ribosomal RNA by using the Southern blotting technique. The resulting bands could be arranged into seven groups, and it was possible to construct a unique physical map of the seven rRNA genes (operons) of the bacterial chromosome. Mapping information obtained on several transducing phages and recombinant plasmids carrying rRNA genes, and mapping data published in the literature helped to determine the final map. The results suggest that phage lambda daroE152 carries a "hybrid" rRNA gene which was probably formed by recombination between two different chromosomal rRNA genes.

MeSH Terms

Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes DNA, Bacterial/metabolism DNA, Recombinant/metabolism Escherichia coli/analysis,genetics Genes Nucleic Acid Hybridization Plasmids RNA, Ribosomal/biosynthesis Transduction, Genetic

Chemicals

DNA, Bacterial DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Boros I

Kiss A

Venetianer P

References (25)

25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.5.1817

Published

1979-00-00

Pages

1817-30

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327813

Subset

External Links

PubMed source DOI: 10.1093/nar/6.5.1817 PMC Full Text View journal details

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Physical map of the seven ribosomal RNA genes of Escherichia coli.

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