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PMID: 3770480 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus.

Gene ·Vol. 44 ·No. 1 ·1986-00-00 ·Pages 139-42

Brand NJ, Fersht AR

Abstract

The valS gene from the thermophile Bacillus stearothermophilus encoding the valyl-tRNA synthetase has been cloned on a 13.8-kb plasmid. The gene product and its kinetic properties are comparable with those of the native enzyme.

MeSH Terms
Amino Acyl-tRNA Synthetases/genetics Cloning, Molecular Enzyme Stability Genes Genes, Bacterial Geobacillus stearothermophilus/enzymology,genetics Kinetics Plasmids Valine-tRNA Ligase/genetics,metabolism
Chemicals
Amino Acyl-tRNA Synthetases Valine-tRNA Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brand N J
Fersht A R
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(86)90053-3
Published
1986-00-00
Pages
139-42
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
External Links
PubMed source DOI: 10.1016/0378-1119(86)90053-3 View journal details
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