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PMID: 3767947 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evaluation of the actin filament length from the time course of the depolymerization process.

Biochemical and biophysical research communications ·Vol. 139 ·No. 1 ·1986-08-29 ·Pages 109-14

Grazi E, Trombetta G

Abstract

When pyrenyl-labelled actin, at intermediate stages of polymerization, is diluted in the polymerization buffer, the decrease of fluorescence takes place stepwise through pseudo zero order reactions of decreasing rate. It is shown that the analysis of the kinetic course of the reaction allows the evaluation of the length of the actin filaments.

MeSH Terms
Actins Animals Fluorescence Kinetics Polymers Rabbits
Chemicals
Actins Polymers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grazi E
Trombetta G
Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
0006-291X
DOI
10.1016/s0006-291x(86)80086-9
Published
1986-08-29
Pages
109-14
Language
English
Region
United States
NLM ID
0372516
Subset
IM
External Links
PubMed source DOI: 10.1016/s0006-291x(86)80086-9 View journal details
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