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PMID: 3760194 Published · ppublish English Journal Article

Binding of fibrinogen to human monocytes.

The Journal of clinical investigation ·Vol. 78 ·No. 4 ·1986-10-00 ·Pages 968-76

Altieri DC, Mannucci PM, Capitanio AM

Abstract

The interaction of fibrinogen with monocytes was studied. After stimulation with ADP (10 microM) or thrombin (1 U/ml), platelet-free suspensions of human monocytes bind 125I-fibrinogen with two different affinities in a specific and Ca2+-dependent reaction with saturation at 5.80-7.35 X 10(-7) M of added protein. The binding of fibrinogen to specific receptors on monocytes induces the procoagulant activity of these cells. Thrombasthenic cells or normal monocytes preincubated with a monoclonal antibody to the platelet glycoprotein IIb/IIIa complex (10E5) do not bind fibrinogen and have no procoagulant activity. Metabolic studies with [35S]methionine revealed that cultured monocytes actually synthesize a surface antigen precipitated by 10E5 antibody as a major band with 92,000 relative molecular weight. Our data indicate that monocytes express receptors for fibrinogen only in part related to the platelet glycoprotein IIb/IIIa complex. Furthermore, the binding of fibrinogen to monocytes enhances the cooperation of these cells in hemostasis.

MeSH Terms
Adenosine Diphosphate/pharmacology Electrophoresis, Polyacrylamide Gel Fibrinogen/metabolism Humans Kinetics Methionine/metabolism Microscopy, Electron Molecular Weight Monocytes/metabolism Platelet Membrane Glycoproteins/metabolism Thrombin/pharmacology
Chemicals
Platelet Membrane Glycoproteins Adenosine Diphosphate Fibrinogen Methionine Thrombin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Altieri D C
Mannucci P M
Capitanio A M
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48 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112687
Published
1986-10-00
Pages
968-76
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423733
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI112687 PMC Full Text View journal details
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