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PMID: 3755421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pathogenesis of Shigella diarrhea: rabbit intestinal cell microvillus membrane binding site for Shigella toxin.

Infection and immunity ·Vol. 53 ·No. 2 ·1986-08-00 ·Pages 372-7

Fuchs G, Mobassaleh M, Donohue-Rolfe A, Montgomery RK, Grand RJ, Keusch GT

Abstract

This study examined the binding of purified 125I-labeled shigella toxin to rabbit jejunal microvillus membranes (MVMs). Toxin binding was concentration dependent, saturable, reversible, and specifically inhibited by unlabeled toxin. The calculated number of toxin molecules bound at 4 degrees C was 7.9 X 10(10) (3 X 10(10) to 2 X 10(11))/micrograms of MVM protein or 1.2 X 10(6) per enterocyte. Scatchard analysis showed the binding site to be of a single class with an equilibrium association constant, K, of 4.7 X 10(9) M-1 at 4 degrees C. Binding was inversely related to the temperature of incubation. A total of 80% of the labeled toxin binding at 4 degrees C dissociated from MVM when the temperature was raised to 37 degrees C, but reassociated when the temperature was again brought to 4 degrees C. There was no structural or functional change of MVM due to toxin as monitored by electron microscopy or assay of MVM sucrase activity. These studies demonstrate a specific binding site for shigella toxin on rabbit MVMs. The physiological relevance of this receptor remains to be determined.

MeSH Terms
Animals Bacterial Toxins/metabolism Dysentery, Bacillary/etiology HeLa Cells/analysis Humans In Vitro Techniques Intestines/analysis,drug effects Iodine Radioisotopes Microvilli/analysis Rabbits Receptors, Cell Surface Receptors, Immunologic/analysis Shiga Toxins Temperature
Chemicals
Bacterial Toxins Iodine Radioisotopes Receptors, Cell Surface Receptors, Immunologic Shiga Toxins shigella toxin receptor
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fuchs G
Mobassaleh M
Donohue-Rolfe A
Montgomery R K
Grand R J
Keusch G T
References (34)
34 references, click to expand
  1. Statistical limits in Scatchard analysis.
    J Biol Chem. 1983 Nov 10;258(21):12865-7 PMID: 6313679
  2. Binding of Clostridium perfringens [125I]enterotoxin to rabbit intestinal cells.
    Biochemistry. 1980 Oct 14;19(21):4801-7 PMID: 6252960
  3. Intestinal lactase synthesis during postnatal development in the rat.
    Pediatr Res. 1985 Sep;19(9):956-62 PMID: 3931041
  4. Pathogenesis of shigella diarrhea. XI. Isolation of a shigella toxin-binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramide.
    J Exp Med. 1986 Jun 1;163(6):1391-404 PMID: 3519828
  5. Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli.
    Infect Immun. 1983 May;40(2):675-83 PMID: 6341244
  6. Receptor-mediated entry of diphtheria toxin into monkey kidney (Vero) cells: electron microscopic evaluation.
    Infect Immun. 1985 Dec;50(3):721-7 PMID: 4066029
  7. Specific membrane receptors: pathogenetic and therapeutic implications in infectious diseases.
    Rev Infect Dis. 1979 May-Jun;1(3):517-29 PMID: 399364
  8. The pathogenesis of Shigella diarrhea. VI. Toxin and antitoxin in Shigella flexneri and Shigella sonnei infections in humans.
    J Infect Dis. 1977 Apr;135(4):552-6 PMID: 323376
  9. Shigellosis due to Shigella dysenteriae. 1. Relative importance of mucosal invasion versus toxin production in pathogenesis.
    J Infect Dis. 1972 Nov;126(5):523-30 PMID: 4197755
  10. Intracellular processing of disaccharidases: the effect of actinomycin D.
    Biochim Biophys Acta. 1971 Feb 28;261(2):341-52 PMID: 5144277
  11. Interaction of cholera toxin with rat intestinal brush border membranes. Relative roles of gangliosides and galactoproteins as toxin receptors.
    J Biol Chem. 1981 Aug 25;256(16):8724-31 PMID: 7263681
  12. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  13. Fate of tetanus toxin bound to the surface of primary neurons in culture: evidence for rapid internalization.
    J Cell Biol. 1985 May;100(5):1499-507 PMID: 3988797
  14. Pathophysiology of Shigella diarrhea in the rhesus monkey: intestinal transport, morphological, and bacteriological studies.
    Gastroenterology. 1975 Feb;68(2):270-8 PMID: 1090480
  15. Pathogenesis of shigella diarrhea. Serum anticytotoxin antibody response produced by toxigenic and nontoxigenic Shigella dysenteriae 1.
    J Clin Invest. 1976 Jan;57(1):194-202 PMID: 1107354
  16. Comparison of secretory and histological effects of shigella and cholera enterotoxins in rabbit jejunum.
    Gastroenterology. 1975 Feb;68(2):309-17 PMID: 1090481
  17. Binding of Escherichia coli heat-stable enterotoxin to rat intestinal cells and brush border membranes.
    Infect Immun. 1984 Feb;43(2):622-30 PMID: 6537947
  18. Synthesis and intracellular processing of sucrase-isomaltase in rat jejunum.
    Gastroenterology. 1985 Feb;88(2):531-8 PMID: 3917419
  19. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems.
    Biochim Biophys Acta. 1978 Jan 4;506(1):136-54 PMID: 620021
  20. Pathogenesis of Shigella dysenteriae 1 (Shiga) dysentery.
    J Infect Dis. 1973 Mar;127(3):261-70 PMID: 4631877
  21. Pathogenesis of shigella diarrhea: evidence for an N-linked glycoprotein shigella toxin receptor and receptor modulation by beta-galactosidase.
    J Infect Dis. 1986 Feb;153(2):238-48 PMID: 3003205
  22. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
    Biochem J. 1973 Jul;133(3):529-39 PMID: 4733239
  23. Shigella toxin and the pathogenesis of shigellosis.
    Ciba Found Symp. 1985;112:193-214 PMID: 3847336
  24. Statistical characterization of the random errors in the radioimmunoassay dose--response variable.
    Clin Chem. 1976 Mar;22(3):350-8 PMID: 175978
  25. The asialoglycoprotein receptor internalizes and recycles independently of the transferrin and insulin receptors.
    Cell. 1983 Jan;32(1):267-75 PMID: 6297785
  26. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  27. Mechanism of action of cholera toxin: effect of receptor density and multivalent binding on activation of adenylate cyclase.
    J Membr Biol. 1980;54(1):51-60 PMID: 6259358
  28. Biological properties of Shigella flexneri 2A toxin and its serological relationship to Shigella dysenteriae 1 toxin.
    Infect Immun. 1977 Mar;15(3):796-8 PMID: 323142
  29. Pathogenesis of Shigella diarrhea. IX. Simplified high yield purification of Shigella toxin and characterization of subunit composition and function by the use of subunit-specific monoclonal and polyclonal antibodies.
    J Exp Med. 1984 Dec 1;160(6):1767-81 PMID: 6392471
  30. Interaction of Shigella shigae cytotoxin with receptors on sensitive and insensitive cells.
    J Recept Res. 1980;1(2):199-213 PMID: 6895387
  31. Interaction of Vibrio cholerae enterotoxin with cell membranes.
    Biochemistry. 1973 Aug 28;12(18):3547-58 PMID: 4731191
  32. Quantitative microassay in cell culture for enterotoxin of Shigella dysenteriae.
    J Infect Dis. 1972 May;125(5):539-41 PMID: 4554110
  33. The preparation of isolated intestinal crypt cells.
    Exp Cell Res. 1969 May;55(2):257-60 PMID: 4977181
  34. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development.
    Microbiol Rev. 1983 Dec;47(4):510-50 PMID: 6363898
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.53.2.372-377.1986
Published
1986-08-00
Pages
372-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260885
Subset
IM
Grants
NIADDK NIH HHS · AM 07471 · United States
NIADDK NIH HHS · AM 32658 · United States
NIADDK NIH HHS · AM 39428 · United States
External Links
PubMed source DOI: 10.1128/iai.53.2.372-377.1986 PMC Full Text View journal details
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