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PMID: 3754588 Published · ppublish English Comparative Study Journal Article

Matrix genes of measles virus and canine distemper virus: cloning, nucleotide sequences, and deduced amino acid sequences.

Journal of virology ·Vol. 58 ·No. 2 ·1986-05-00 ·Pages 408-16

Bellini WJ, Englund G, Richardson CD, Rozenblatt S, Lazzarini RA

Abstract

The nucleotide sequences encoding the matrix (M) proteins of measles virus (MV) and canine distemper virus (CDV) were determined from cDNA clones containing these genes in their entirety. In both cases, single open reading frames specifying basic proteins of 335 amino acid residues were predicted from the nucleotide sequences. Both viral messages were composed of approximately 1,450 nucleotides and contained 400 nucleotides of presumptive noncoding sequences at their respective 3' ends. MV and CDV M-protein-coding regions were 67% homologous at the nucleotide level and 76% homologous at the amino acid level. Only chance homology was observed in the 400-nucleotide trailer sequences. Comparisons of the M protein sequences of MV and CDV with the sequence reported for Sendai virus (B. M. Blumberg, K. Rose, M. G. Simona, L. Roux, C. Giorgi, and D. Kolakofsky, J. Virol. 52:656-663; Y. Hidaka, T. Kanda, K. Iwasaki, A. Nomoto, T. Shioda, and H. Shibuta, Nucleic Acids Res. 12:7965-7973) indicated the greatest homology among these M proteins in the carboxyterminal third of the molecule. Secondary-structure analyses of this shared region indicated a structurally conserved, hydrophobic sequence which possibly interacted with the lipid bilayer.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Distemper Virus, Canine/genetics Genes Genes, Viral Measles virus/genetics Protein Conformation RNA, Viral/genetics Sequence Homology, Nucleic Acid Viral Matrix Proteins Viral Proteins/genetics
Chemicals
RNA, Viral Viral Matrix Proteins Viral Proteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bellini W J
Englund G
Richardson C D
Rozenblatt S
Lazzarini R A
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30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.2.408-416.1986
Published
1986-05-00
Pages
408-16
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252926
Subset
IM
Databases
GENBANK
M12668, M12669
External Links
PubMed source DOI: 10.1128/JVI.58.2.408-416.1986 PMC Full Text View journal details
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