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PMID: 3748820 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging.

Nucleic acids research ·Vol. 14 ·No. 16 ·1986-08-26 ·Pages 6711-9

Simpson VJ, Johnson TE, Hammen RF

Abstract

DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restriction endonuclease isoschizomers, HpaII and MspI, were used to digest genomic DNA after CsCl purification and failed to detect any 5' cytosine methylation at any age. We conclude that C. elegans does not contain detectable (0.01 mole percent) levels of 5-methylcytosine.

MeSH Terms
5-Methylcytosine Aging Animals Caenorhabditis/genetics,growth & development Chromatography, High Pressure Liquid Crosses, Genetic Cytosine/analogs & derivatives,analysis DNA/isolation & purification Nucleic Acid Hybridization Species Specificity
Chemicals
5-Methylcytosine Cytosine DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Simpson V J
Johnson T E
Hammen R F
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19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.16.6711
Published
1986-08-26
Pages
6711-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311675
Subset
IM
Grants
NIA NIH HHS · AG 05720 · United States
NIA NIH HHS · N01-AG-9-2113 · United States
External Links
PubMed source DOI: 10.1093/nar/14.16.6711 PMC Full Text View journal details
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