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PMID: 3748802 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of a Xenopus laevis mitochondrial protein with a high affinity for supercoiled DNA.

Nucleic acids research ·Vol. 14 ·No. 15 ·1986-08-11 ·Pages 5969-80

Mignotte B, Barat M

Abstract

A DNA binding protein of 31 Kd -mtDBPC- has been isolated from X. laevis oocyte mitochondria. It is present in large amounts in the organelle and does not show any enzymatic activity. Its binding to the superhelical form of a DNA is higher than for any other form, or for RNA. No sequence specificity could be found for any mtDNA fragments tested, including both origins of replication. It is able to introduce superhelical turns into relaxed circular DNA in the presence of a topoisomerase I activity. It could be a component of the mitochondrial nucleoids.

MeSH Terms

Animals DNA, Mitochondrial/metabolism DNA, Superhelical/metabolism DNA-Binding Proteins/isolation & purification,metabolism Female Mitochondria/analysis Nucleic Acid Conformation Oocytes/analysis Xenopus laevis/metabolism

Chemicals

DNA, Mitochondrial DNA, Superhelical DNA-Binding Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mignotte B

Barat M

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.15.5969

Published

1986-08-11

Pages

5969-80

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311615

Subset

External Links

PubMed source DOI: 10.1093/nar/14.15.5969 PMC Full Text View journal details

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Characterization of a Xenopus laevis mitochondrial protein with a high affinity for supercoiled DNA.

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