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PMID: 3745277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The alteration of myosin isoform compartmentation in specific mutants of Caenorhabditis elegans.

The Journal of cell biology ·Vol. 103 ·No. 3 ·1986-09-00 ·Pages 985-93

Epstein HF, Ortiz I, Mackinnon LA

Abstract

Myosin isoforms A and B are located at the surface of the central and polar regions, respectively, of thick filaments in body muscle cells of Caenorhabditis elegans, whereas paramyosin and a distinct core structure comprise the backbones of these filaments. Thick filaments and related structures were isolated from nematode mutants that have altered thick filament protein compositions. These mutant filaments and their complexes with specific antibodies were studied by electron microscopy to determine the distribution of the two myosins. The compartmentation of the two myosin isoforms in body wall muscle thick filaments depends not only upon the intrinsic properties of the myosins but their interactions with other components such as paramyosin and their relative quantities determined by synthesis.

MeSH Terms
Animals Caenorhabditis/anatomy & histology,genetics Cell Compartmentation Muscles/analysis Myosins/analysis Tropomyosin/analysis
Chemicals
Tropomyosin Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Epstein H F
Ortiz I
Mackinnon L A
References (20)
20 references, click to expand
  1. A protein that binds specifically to the M-line of skeletal muscle is identified as the muscle form of creatine kinase.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):702-5 PMID: 4197625
  2. Myosin and paramyosin are organized about a newly identified core structure.
    J Cell Biol. 1985 Mar;100(3):904-15 PMID: 3972901
  3. Paramyosin of Caenorhabditis elegans.
    J Mol Biol. 1974 Dec 5;90(2):285-90 PMID: 4217841
  4. A mutant affecting the heavy chain of myosin in Caenorhabditis elegans.
    J Mol Biol. 1974 Dec 5;90(2):291-300 PMID: 4453018
  5. Myosin and paramyosin of Caenorhabditis elegans: biochemical and structural properties of wild-type and mutant proteins.
    Cell. 1977 Apr;10(4):709-19 PMID: 140764
  6. Two homogeneous myosins in body-wall muscle of Caenorhabditis elegans.
    Cell. 1977 Apr;10(4):721-8 PMID: 862026
  7. Identification of the structural gene for a myosin heavy-chain in Caenorhabditis elegans.
    J Mol Biol. 1977 Jul;114(1):133-40 PMID: 909083
  8. Indirect suppression in Caenorhabditis elegans.
    Genetics. 1978 Jun;89(2):299-314 PMID: 669254
  9. A suppressor mutation in the nematode acting on specific alleles of many genes.
    Nature. 1978 Oct 26;275(5682):715-9 PMID: 703836
  10. Myosins exist as homodimers of heavy chains: demonstration with specific antibody purified by nematode mutant myosin affinity chromatography.
    Cell. 1978 Oct;15(2):405-11 PMID: 82486
  11. Immunocytochemical localization of two myosins within the same muslce cells in Caenorhabditis elegans.
    Cell. 1978 Oct;15(2):413-9 PMID: 363276
  12. Coordinate synthesis of two myosins in wild-type and mutant nematode muscle during larval development.
    Cell. 1978 Oct;15(2):421-8 PMID: 719748
  13. Muscle development in Caenorhabditis elegans: mutants exhibiting retarded sarcomere construction.
    Cell. 1978 Nov;15(3):751-62 PMID: 728988
  14. Mutants altering coordinate synthesis of specific myosins during nematode muscle development.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):852-6 PMID: 6928685
  15. Paramyosin is necessary for determination of nematode thick filament length in vivo.
    Cell. 1980 Dec;22(3):747-55 PMID: 7193096
  16. Molecular analysis of the unc-54 myosin heavy-chain gene of Caenorhabditis elegans.
    Nature. 1981 Jun 4;291(5814):386-90 PMID: 6264304
  17. A second informational suppressor, SUP-7 X, in Caenorhabditis elegans.
    Genetics. 1981 Feb;97(2):307-25 PMID: 7274656
  18. Differential localization of two myosins within nematode thick filaments.
    Cell. 1983 Sep;34(2):477-90 PMID: 6352051
  19. The relationship between stress fiber-like structures and nascent myofibrils in cultured cardiac myocytes.
    J Cell Biol. 1984 Dec;99(6):2268-78 PMID: 6438115
  20. The genetics of Caenorhabditis elegans.
    Genetics. 1974 May;77(1):71-94 PMID: 4366476
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.3.985
Published
1986-09-00
Pages
985-93
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114276
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.103.3.985 PMC Full Text View journal details
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