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PMID: 3745276 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Studies on the transcription, translation, and structure of alpha-actinin in Dictyostelium discoideum.

The Journal of cell biology ·Vol. 103 ·No. 3 ·1986-09-00 ·Pages 969-75

Witke W, Schleicher M, Lottspeich F, Noegel A

Abstract

A clone coding for the F-actin cross-linking protein alpha-actinin was obtained by screening a genomic library of Dictyostelium discoideum DNA in lambda gt11 with monoclonal antibodies specific for Dictyostelium alpha-actinin. The 1.2-kilobase (kb) genomic clone was confirmed as containing part of the alpha-actinin gene by comparing its nucleotide sequence with the amino acid sequence of tryptic peptides from purified alpha-actinin. The clone recognized a 3.0-kb message in a Northern blot. Hybridization to RNA isolated from different developmental stages of several D. discoideum strains indicated that the mRNA content increased during early development. A similar result was obtained when the alpha-actinin content of the cells was followed by Western blot analysis. Hybridization of the clone to DNA from different wild-type strains of D. discoideum indicated a polymorphism on the DNA level that coincided with a polymorphism on the protein level. The data suggest continuous transcription of the alpha-actinin gene throughout the development of D. discoideum, up- and down-regulation of the levels of alpha-actinin mRNA and protein with maximum levels at the onset of aggregation, and a high diversity of alpha-actinin at the DNA and protein level among different D. discoideum strains. The structural data make it conceivable that the highly conserved nature of alpha-actinin resides only at the functional sites, whereas the helical portions of the alpha-actinin molecule allow a higher level of diversity throughout evolution.

MeSH Terms
Actinin/analysis,genetics Amino Acid Sequence Base Sequence DNA, Fungal/analysis Dictyostelium/analysis,genetics Polymorphism, Genetic Protein Biosynthesis Protein Conformation RNA, Fungal/analysis RNA, Messenger/analysis Recombinant Proteins/analysis,genetics Transcription, Genetic
Chemicals
DNA, Fungal RNA, Fungal RNA, Messenger Recombinant Proteins Actinin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Witke W
Schleicher M
Lottspeich F
Noegel A
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39 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.3.969
Published
1986-09-00
Pages
969-75
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114308
Subset
IM
Databases
GENBANK
X04324
External Links
PubMed source DOI: 10.1083/jcb.103.3.969 PMC Full Text View journal details
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