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PMID: 3744905 Published · ppublish English Journal Article

Ultrastructural localization of calcium in the CNS of vertebrates.

Histochemistry ·Vol. 85 ·No. 3 ·1986-00-00 ·Pages 231-9

Probst W

Abstract

The ultrastructural localization of calcium in synaptic areas of the CNS of fish was investigated. Prefixation with phosphate-buffered glutaraldehyde followed by post-fixation with osmium/potassium-bichromate was used to precipitate and visualize endogenous calcium without the addition of external calcium. The presence of calcium in the electron-dense precipitates produced using this method was demonstrated by electron spectroscopic imaging using a Zeiss EM-902 transmission electron microscope, and in various control experiments using the calcium chelator EGTA. In the optic tectum of fish, electron dense precipitates containing calcium were found not only in intracellular compartments, e.g. the smooth endoplasmic reticulum, mitochondria and synaptic vesicles, but also at extracellular locations, particularly in synaptic clefts. In the extracellular sites, only chelate complexes of ionic calcium were found. This would seem to be in agreement with electrophysiological and biochemical data reported in earlier studies. Thus, using the present method, it should be possible to obtain further ultrastructural information concerning the mechanisms of synaptic transmission.

MeSH Terms
Animals Calcium/analysis Carps Indicators and Reagents Microscopy, Electron/methods Superior Colliculi/ultrastructure Synapses/ultrastructure
Chemicals
Indicators and Reagents Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Probst W
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44 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00494809
Published
1986-00-00
Pages
231-9
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00494809
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