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PMID: 374352 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Screening for amino acid pool mutants of Neurospora and yeasts: replica-printing technique.

Journal of bacteriology ·Vol. 137 ·No. 3 ·1979-03-00 ·Pages 1437-8

Cramer CL, Davis RH

Abstract

A replica-printing technique has been developed for permeabilized colonies of Neurospora and yeast by which endogenous amino acid pools are visualized. By the use of cation-exchange paper, the method has detected Neurospora mutants unable to store the large pools of basic amino acids characteristic of wild type. Mutants of this sort were sought to study the function of the fungal vacuole.

MeSH Terms

Amino Acids/genetics Microbiological Techniques Mutation Neurospora/genetics,isolation & purification

Chemicals

Amino Acids

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cramer C L

Davis R H

References (3)

3 references, click to expand

Intracellular localization of ornithine and arginine pools in Neurospora.
J Biol Chem. 1973 Aug 10;248(15):5409-13 PMID: 4272157
Utilization of exogenous and endogenous ornithine by Neurospora crassa.
J Bacteriol. 1968 Aug;96(2):389-95 PMID: 5674053
Isolation of Escherichia coli mutants defective in enzymes of membrane lipid synthesis.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2274-8 PMID: 49056

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.137.3.1437-1438.1979

Published

1979-03-00

Pages

1437-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC218333

Subset

External Links

PubMed source DOI: 10.1128/jb.137.3.1437-1438.1979 PMC Full Text View journal details

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Screening for amino acid pool mutants of Neurospora and yeasts: replica-printing technique.

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