Progress-curve analysis in enzyme kinetics. Numerical solution of integrated rate equations.

The Biochemical journal ·Vol. 235 ·No. 2 ·1986-04-15 ·Pages 613-5

Duggleby RG

Abstract

Progress curves of enzyme-catalysed reactions are described by equations of a type that precludes direct calculation of the extent of reaction at any time. Previously, such equations have been solved by the Newton-Raphson method, but this procedure may fail when based upon the usual formulae. An alternative formulation is proposed that is both quicker and more robust.

MeSH Terms

Enzymes/metabolism Kinetics Methods Models, Chemical

Chemicals

Enzymes

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Duggleby R G

References (5)

5 references, click to expand

Statistical estimations in enzyme kinetics. The integrated Michaelis equation.
Eur J Biochem. 1974 Apr 1;43(2):377-8 PMID: 4838986
Integrated steady state rate equations and the determination of individual rate constants.
J Biol Chem. 1975 Jun 25;250(12):4696-701 PMID: 1141225
Analysis of progress curves for a highly concentrated Michaelian enzyme in the presence or absence of product inhibition.
Biochem J. 1985 Oct 1;231(1):65-74 PMID: 4062893
Progress curve analysis in enzyme kinetics: model discrimination and parameter estimation.
Biochim Biophys Acta. 1978 Oct 12;526(2):398-409 PMID: 718944
The analysis of progress curves for enzyme-catalysed reactions by non-linear regression.
Biochim Biophys Acta. 1977 Apr 12;481(2):297-312 PMID: 870047

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2350613

Published

1986-04-15

Pages

613-5

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1146728

Subset

External Links

PubMed source DOI: 10.1042/bj2350613 PMC Full Text View journal details

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Progress-curve analysis in enzyme kinetics. Numerical solution of integrated rate equations.

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