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PMID: 3740412 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions.

Analytical biochemistry ·Vol. 156 ·No. 1 ·1986-07-00 ·Pages 220-2

Staros JV, Wright RW, Swingle DM

Abstract

Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.

MeSH Terms
Carbodiimides Glycine Hemocyanins Solubility Succinimides Water
Chemicals
Carbodiimides Succinimides Water N-hydroxysulfosuccinimide Hemocyanins Glycine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Staros J V
Wright R W
Swingle D M
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(86)90176-4
Published
1986-07-00
Pages
220-2
Language
English
Region
United States
NLM ID
0370535
Subset
IM
Grants
NIADDK NIH HHS · AM25489 · United States
NIADDK NIH HHS · AM31880 · United States
NIADDK NIH HHS · T35 AM07383 · United States
External Links
PubMed source DOI: 10.1016/0003-2697(86)90176-4 View journal details
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