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PMID: 3735493 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and protein overproduction of bacteriophage T4 thioredoxin.

Journal of virology ·Vol. 59 ·No. 3 ·1986-09-00 ·Pages 759-60

LeMaster DM

Abstract

The bacteriophage T4 thioredoxin gene was cloned and physically mapped to 47.6 kilobases from the reference BamHI site. The DNA sequence is consistent with that reported from earlier protein sequence studies. The gene was subcloned into a lambda pL overexpression vector which allowed for the isolation of approximately 5 mg/liter.

MeSH Terms

Bacterial Proteins/genetics Base Sequence Cloning, Molecular Genes, Viral T-Phages/genetics,metabolism Thioredoxins/biosynthesis,genetics Viral Proteins/biosynthesis,genetics

Chemicals

Bacterial Proteins Viral Proteins Thioredoxins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

LeMaster D M

References (15)

15 references, click to expand

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Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
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The use of pKc30 and its derivatives for controlled expression of genes.
Methods Enzymol. 1983;101:123-38 PMID: 6310317
A physical map of bacteriophage T4 including the positions of strong promoters and terminators recognized in vitro.
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Conformational and functional similarities between glutaredoxin and thioredoxins.
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.59.3.759-760.1986

Published

1986-09-00

Pages

759-60

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253257

Subset

Grants

NIGMS NIH HHS · GM22778 · United States

Databases

GENBANK

M13894

External Links

PubMed source DOI: 10.1128/JVI.59.3.759-760.1986 PMC Full Text View journal details

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Nucleotide sequence and protein overproduction of bacteriophage T4 thioredoxin.

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