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PMID: 3728976 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Artificial reductant enhancement of the Lowry method for protein determination.

Analytical biochemistry ·Vol. 155 ·No. 2 ·1986-06-00 ·Pages 243-8

Larson E, Howlett B, Jagendorf A

Abstract

Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.

MeSH Terms
Colorimetry Dithiothreitol Indicators and Reagents Molybdenum Oxidation-Reduction Proteins/analysis Spectrophotometry Tungsten Tungsten Compounds
Chemicals
Folin's phenol reagent Indicators and Reagents Proteins Tungsten Compounds Molybdenum Dithiothreitol Tungsten
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Larson E
Howlett B
Jagendorf A
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(86)90432-x
Published
1986-06-00
Pages
243-8
Language
English
Region
United States
NLM ID
0370535
Subset
IM
Grants
NIGMS NIH HHS · GM-14479 · United States
External Links
PubMed source DOI: 10.1016/0003-2697(86)90432-x View journal details
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