Electrolyte effects on the activity of mutant enzymes in vivo and in vitro.

Biochemistry ·Vol. 18 ·No. 7 ·1979-04-03 ·Pages 1386-92

Kohno T, Roth J

Abstract

All temperature-sensitive histidine auxotrophs of Salmonella typhimurium tested are corrected by addition of neutral salts to their growth medium. The correctability seems to result from direct electrolyte effects on mutant protein stability since several of the mutant proteins are also salt correctable in vitro.

MeSH Terms

Alcohol Oxidoreductases/metabolism Ammonium Sulfate/pharmacology Crosses, Genetic Histidine/metabolism Histidinol Kinetics Mutation Salmonella Phages Salmonella typhimurium/enzymology Sodium Chloride/pharmacology Species Specificity Transduction, Genetic

Chemicals

Sodium Chloride Histidine Histidinol Alcohol Oxidoreductases Ammonium Sulfate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kohno T

Roth J

Article Info

Journal

Biochemistry

Abbr.

Biochemistry

ISSN

0006-2960

DOI

10.1021/bi00574a041

Published

1979-04-03

Pages

1386-92

Language

English

Region

United States

NLM ID

0370623

Subset

External Links

PubMed source DOI: 10.1021/bi00574a041 View journal details

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Electrolyte effects on the activity of mutant enzymes in vivo and in vitro.

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