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PMID: 3714445 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A comparison of two different indicators: quin 2 and aequorin in isolated single cells and intact strips of ferret portal vein.

Pflugers Archiv : European journal of physiology ·Vol. 406 ·No. 4 ·1986-04-00 ·Pages 427-9

DeFeo TT, Morgan KG

Abstract

A comparison between the fluorescent indicator quin 2 and the bioluminescent indicator aequorin was performed in the same smooth muscle cell type. Aequorin was loaded into intact strips and quin was loaded into enzymatically isolated single cells from ferret portal vein. Both indicators gave qualitatively the same calcium profiles when the tissue was challenged with agonists. Quin loading caused a dramatic shift to the right in dose response curves to potassium and phenylephrine. The ED50 values for quin loaded cells were significantly different from those for control cells for both agonists. Intracellular calcium levels at rest were not significantly different with quin and aequorin. Cells stimulated with potassium gave significantly different intracellular calcium values with the two indicators suggesting a change in the stimulated steady state level due to the introduction of an additional calcium buffer (quin2) into the cell.

MeSH Terms
Aequorin Aminoquinolines Animals Calcium/metabolism Cell Separation Dose-Response Relationship, Drug Ferrets Luminescent Proteins Microscopy, Fluorescence Portal Vein/cytology,metabolism
Chemicals
Aminoquinolines Luminescent Proteins Aequorin Quin2 Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeFeo T T
Morgan K G
References (17)
17 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00590948
Published
1986-04-00
Pages
427-9
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
Grants
NHLBI NIH HHS · HL31704 · United States
External Links
PubMed source DOI: 10.1007/BF00590948 View journal details
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