Binding characteristics of 3H-prazosin to rat brain alpha-adrenergic receptors.

European journal of pharmacology ·Vol. 55 ·No. 3 ·1979-05-01 ·Pages 323-6

Greengrass P, Bremner R

Abstract

3H-Prazosin binding to membranes from rat brain is saturable, Bmax, 77 fmol/mg protein, of high affinity, KD, 0.28 nM and with a drug specificity indicating that it labels alpha-adrenergic receptors. The ranking of alpha-antagonists for these binding sites suggests that 3H-prazosin may be useful in identifying a subpopulation of alpha-receptors (alpha1) in the central nervous system.

MeSH Terms

Adrenergic alpha-Agonists/metabolism Adrenergic alpha-Antagonists/metabolism Animals Brain/metabolism In Vitro Techniques Male Membranes/metabolism Prazosin/metabolism Protein Binding Quinazolines/metabolism Rats Receptors, Adrenergic/metabolism Receptors, Adrenergic, alpha/metabolism

Chemicals

Adrenergic alpha-Agonists Adrenergic alpha-Antagonists Quinazolines Receptors, Adrenergic Receptors, Adrenergic, alpha Prazosin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Greengrass P

Bremner R

Article Info

Journal

European journal of pharmacology

Abbr.

Eur J Pharmacol

ISSN

0014-2999

DOI

10.1016/0014-2999(79)90202-4

Published

1979-05-01

Pages

323-6

Language

English

Region

Netherlands

NLM ID

1254354

Subset

External Links

PubMed source DOI: 10.1016/0014-2999(79)90202-4 View journal details

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Binding characteristics of 3H-prazosin to rat brain alpha-adrenergic receptors.

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