RNA-RNA interactions in the binding site of protein L24 on 23S ribosomal RNA of E. coli. II. Sequence analysis of the interacting fragments.

Nucleic acids research ·Vol. 5 ·No. 12 ·1978-12-00 ·Pages 4933-47

Krol A, Machatt MA, Branlant C, Ebel JP

Abstract

A ribonucleoprotein complex containing several RNA subfragments from the 5' part of 23S RNA was recovered after digestion of the reconstituted complex between 23S RNA and protein L24. It was suggested in the preceding paper that the RNA subfragments 4B, 10A and 9, which are widely separated in the sequence, strongly interact. These subfragments were previously partially sequenced by the classical fingerprinting methods. Their sequences have now been completed with rapid new RNA sequencing methods. We propose here a base-pairing model showing how these subfragments may interact with one another.

MeSH Terms

Base Sequence Escherichia coli/analysis Oligoribonucleotides/analysis Protein Binding RNA, Ribosomal Ribonucleases Ribosomal Proteins

Chemicals

Oligoribonucleotides RNA, Ribosomal Ribosomal Proteins Ribonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Krol A

Machatt M A

Branlant C

Ebel J P

References (14)

14 references, click to expand

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RNA-RNA interactions in the binding site of protein L24 on 23S ribosomal RNA of Escherichia coli: 1. Evidence for their occurrence between widely separated sequence regions.
Nucleic Acids Res. 1978 Oct;5(10):3503-13 PMID: 364413
Application of a rapid gel method to the sequencing of fragments of 16S ribosomal RNA from Escherichia coli.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.12.4933

Published

1978-12-00

Pages

4933-47

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342799

Subset

External Links

PubMed source DOI: 10.1093/nar/5.12.4933 PMC Full Text View journal details

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RNA-RNA interactions in the binding site of protein L24 on 23S ribosomal RNA of E. coli. II. Sequence analysis of the interacting fragments.

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