Chromosomal location of the mop (groE) gene necessary for bacteriophage morphogenesis in escherichia coli.

The chromosomal location of a host gene, mop (groE), which is essential for the morphogenesis of several bacteriophages in Escherichia coli, was determined by two- and three-factor transductional crosses using phage P1. Cotransduction frequencies beteen mop and other markers were: aspA, 90%; ampA, 77%; frdA, 73%; mel, 24%. The sequence of markers in the corresponding segment (mel to purA; 91.5 to 93.5 min) of the E. coli linkage map was shown to be mel--aspA--mop(groE)--ampA--frdA--pur A.