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PMID: 3689343 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Acute alterations in sodium flux in vitro lead to decreased myofibrillar protein breakdown in rat skeletal muscle.

The Biochemical journal ·Vol. 247 ·No. 1 ·1987-10-01 ·Pages 151-6

Goodman MN

Abstract

Myofibrillar protein breakdown was evaluated by measuring the release of N tau-methylhistidine by isolated rat skeletal muscles or perfused rat muscles in the presence of a variety of agents known to affect Na+ flux. Total cell proteolysis was evaluated simultaneously by measuring tyrosine release by muscles after the inhibition of protein synthesis with cycloheximide. Treatment of muscles with the Na+ ionophore monensin or inhibitors of Na+-K+ ATPase (ouabain, digoxin or vanadate) decreased N tau-methylhistidine release by muscles by 21-35%. A phorbol ester (phorbol 12-myristate 13-acetate) as well as a synthetic diacylglycerol known to activate protein kinase C and a Na+/H+ antiport also decreased N tau-methylhistidine release by muscles. Removal of extracellular Na+ blocked the ability of these agents to attenuate N tau-methylhistidine release by muscles, suggesting that their effectiveness required a change in Na+ flux. In contrast with N tau-methylhistidine release by muscles, these agents, except for monensin, did not effect the release of tyrosine, suggesting that they attenuate specifically the breakdown of myofibrillar proteins. Overall these results indicate a link between Na+ and the regulation of protein breakdown in rat skeletal muscle, whereby an influx of Na+ can result in a decrease in myofibrillar proteolysis. Left unresolved is whether phospholipid hydrolysis is involved in this scheme.

MeSH Terms
Animals Diglycerides/pharmacology Digoxin/pharmacology Male Methylhistidines/metabolism Monensin/pharmacology Muscle Proteins/metabolism Muscles/drug effects Myofibrils/metabolism Ouabain/pharmacology Peptides/pharmacology Rats Rats, Inbred Strains Sodium/metabolism Tetradecanoylphorbol Acetate/pharmacology Vanadates/pharmacology
Chemicals
Diglycerides Methylhistidines Muscle Proteins Peptides Vanadates Ouabain Digoxin 1-oleoyl-2-acetylglycerol Monensin Sodium 3-methylhistidine Tetradecanoylphorbol Acetate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Goodman M N
Department of Medicine, University of California at Davis, Sacramento 95817.
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2470151
Published
1987-10-01
Pages
151-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148382
Subset
IM
Grants
NIADDK NIH HHS · AM 19469 · United States
External Links
PubMed source DOI: 10.1042/bj2470151 PMC Full Text View journal details
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