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PMID: 368586 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The involvement of DNA polymerase I in the postreplication repair of ultraviolet radiation-induced damage in Escherichia coli K-12.

Molecular & general genetics : MGG ·Vol. 167 ·No. 1 ·1978-11-16 ·Pages 37-41

Barfknecht TR, Smith KC

Abstract

A deficiency in DNA polymerase I increased the ultraviolet (UV) radiation sensitivity of a uvrA strain of Escherichia coli K-12 when plated on minimal growth medium. The slope of the survival curve for the uvrA polA strain was 2.0-times greater than that for the uvrA strain. The fluence-dependent yield of unrepaired deoxyribonucleic acid (DNA) parental-strand breaks following UV irradiation and incubation in minimal growth medium was similar in both strains. However, the fluence-dependent yield of unrepaired DNA daughter-strand gaps observed following UV irradiation was 1.8-fold greater in the uvrA polA strain than in the uvrA strain. These results suggest that DNA polymerase I is involved in the filling of at least some daughter-strand gaps during postreplication repair. Also, the uvrA polA strain was sensitized by a post-UV treatment with chloramphenicol (CAP) to a similar extent as was the uvrA strain, indicating that DNA polymerase I is not involved in the CAP-inhibitable pathway of postreplication repair.

MeSH Terms
DNA Polymerase I/metabolism DNA Repair DNA Replication DNA-Directed DNA Polymerase/metabolism Escherichia coli/radiation effects Phenotype Radiation Tolerance Ultraviolet Rays
Chemicals
DNA Polymerase I DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barfknecht T R
Smith K C
References (27)
27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270319
Published
1978-11-16
Pages
37-41
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270319
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